製品の概要

  • 製品名
    Anti-Cdc25B (phospho S323) antibody
    Cdc25B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cdc25B (phospho S323)
  • 特異性
    Detects endogenous levels of Cdc25B only when phosphorylated at serine 323
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from Human Cdc25B around the phosphorylation site of serine 323 (SPSPMP)

  • ポジティブ・コントロール
    • NIH3T3 cell extract treated with PMA

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/20000.
WB 1/300 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

ターゲット情報

  • 機能
    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
  • 配列類似性
    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • 翻訳後修飾
    Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
  • 細胞内局在
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI604853 antibody
    • Cdc 25B antibody
    • Cdc25b antibody
    • CDC25HU2 antibody
    • Cdc25m2 antibody
    • Cell division cycle 25 homolog B antibody
    • Cell division cycle 25B antibody
    • Cell division cycle 25B isoform 1 antibody
    • Cell division cycle 25B isoform 2 antibody
    • Cell division cycle 25B isoform 3 antibody
    • Cell division cycle 25B isoform 4 antibody
    • Cell division cycle 25B isoform 5 antibody
    • Dual specificity phosphatase Cdc25B antibody
    • EC 3.1.3.48 antibody
    • M phase inducer phosphatase 2 antibody
    • M-phase inducer phosphatase 2 antibody
    • MPIP2_HUMAN antibody
    see all

画像

  • All lanes : Anti-Cdc25B (phospho S323) antibody (ab53103) at 1/300 dilution

    Lane 1 : NIH/3T3 cell extract treated with PMA (125ng/ml, 30 mins)
    Lane 2 : NIH/3T3 cell extract treated with PMA (125ng/ml, 30 mins) and the immunogenic phosphopeptide.


    Predicted band size : 65 kDa
    Observed band size : 65 kDa

プロトコール

参考文献

This product has been referenced in:
  • Xu X  et al. A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity. Nat Commun 8:14059 (2017). WB ; Human . Read more (PubMed: 28094252) »
  • Zhao X  et al. Successive recruitment of p-CDC25B-Ser351 and p-cyclin B1-Ser123 to centrosomes contributes to the release of mouse oocytes from prophase I arrest. Dev Dyn 244:110-21 (2015). Read more (PubMed: 25349079) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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