Anti-Cdc25B (phospho S187) 抗体 (ab59986)

製品の概要

  • 製品名
    Anti-Cdc25B (phospho S187) antibody
    Cdc25B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cdc25B (phospho S187)
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phosphpeptide (Human) corresponding to amino acid residues surrounding serine 187, conjugated to KLH

  • ポジティブ・コントロール
    • SK-BR-3 cell lysate Human hepatocarcinoma tissue

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
  • 配列類似性
    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • 翻訳後修飾
    Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
  • 細胞内局在
    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI604853 antibody
    • Cdc 25B antibody
    • Cdc25b antibody
    • CDC25HU2 antibody
    • Cdc25m2 antibody
    • Cell division cycle 25 homolog B antibody
    • Cell division cycle 25B antibody
    • Cell division cycle 25B isoform 1 antibody
    • Cell division cycle 25B isoform 2 antibody
    • Cell division cycle 25B isoform 3 antibody
    • Cell division cycle 25B isoform 4 antibody
    • Cell division cycle 25B isoform 5 antibody
    • Dual specificity phosphatase Cdc25B antibody
    • EC 3.1.3.48 antibody
    • M phase inducer phosphatase 2 antibody
    • M-phase inducer phosphatase 2 antibody
    • MPIP2_HUMAN antibody
    see all

画像

  • Anti-Cdc25B (phospho S187) antibody (ab59986) at 1/100 dilution + SK-BR-3 cell lysate

    Predicted band size : 65 kDa
    Observed band size : 65 kDa
    Additional bands at : 100 kDa,50 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of formalin fixed and paraffin embedded human hepatocarcinoma tissue labelled with ab59986 at 1/50-1/100 dilution followed by peroxidase-conjugation to the secondary antibody and AEC staining.

参考文献

ab59986 has not yet been referenced specifically in any publications.

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