製品の概要

  • 製品名Anti-Cdc25B antibody [EPR3459(2)]
    Cdc25B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3459(2)] to Cdc25B
  • アプリケーション適用あり: WB, IPmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human Cdc25B

  • ポジティブ・コントロール
    • K562 cell lysate, U937 cell lysate and THP-1 cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124819 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IP 1/10 - 1/100.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
    • 配列類似性Belongs to the MPI phosphatase family.
      Contains 1 rhodanese domain.
    • 翻訳後修飾Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
    • 細胞内局在Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AI604853 antibody
      • Cdc 25B antibody
      • Cdc25b antibody
      • CDC25HU2 antibody
      • Cdc25m2 antibody
      • Cell division cycle 25 homolog B antibody
      • Cell division cycle 25B antibody
      • Cell division cycle 25B isoform 1 antibody
      • Cell division cycle 25B isoform 2 antibody
      • Cell division cycle 25B isoform 3 antibody
      • Cell division cycle 25B isoform 4 antibody
      • Cell division cycle 25B isoform 5 antibody
      • Dual specificity phosphatase Cdc25B antibody
      • EC 3.1.3.48 antibody
      • M phase inducer phosphatase 2 antibody
      • M-phase inducer phosphatase 2 antibody
      • MPIP2_HUMAN antibody
      see all

    Anti-Cdc25B antibody [EPR3459(2)] 画像

    • All lanes : Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : THP-1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 64 kDa
      Observed band size : 64 kDa

    Anti-Cdc25B antibody [EPR3459(2)] (ab124819) 使用論文

    ab124819 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab124819.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"