製品の概要

  • 製品名Anti-Cdc25A antibody (Agarose)
    Cdc25A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cdc25A (Agarose)
  • 標識Agarose
  • アプリケーション適用あり: IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide that represents a portion of human Cdc25a encoded by exon 7.

  • 特記事項100ug of antibody is conjugated to 200ul of agarose and provided as a 50% slurry in a total of 400ul volume.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP
  • 追加情報IP: Use at a concentration of 15 - 25 µl/0.1 - 1mg of protein lysate or extract.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
    • 配列類似性Belongs to the MPI phosphatase family.
      Contains 1 rhodanese domain.
    • ドメインThe phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.
    • 翻訳後修飾Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
      Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cdc 25a antibody
      • CDC25A antibody
      • CDC25A2 antibody
      • CDC25A2 CAG isoform antibody
      • Cell division cycle 25 homolog A (S. pombe) antibody
      • Cell division cycle 25A antibody
      • Cell division cycle 25A isoform a antibody
      • Cell division cycle 25A isoform b antibody
      • D9Ertd393e antibody
      • Dual specificity phosphatase Cdc25A antibody
      • M phase inducer phosphatase 1 antibody
      • M-phase inducer phosphatase 1 antibody
      • MGC115549 antibody
      • MPIP1_HUMAN antibody
      see all

    Anti-Cdc25A antibody (Agarose) (ab19203) 使用論文

    ab19203 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"