製品の概要

  • 製品名Anti-Cdc20 antibody
    Cdc20 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cdc20
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 50 and 100 of Human Cdc20 (NP_001246.2).

  • ポジティブ・コントロール
    • Human Anaplastic Thyroid Carcinoma, Breast Carcinoma, Colon Carcinoma and Prostate Carcinoma tissues. Mouse teratoma tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab86104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
    • パスウェイProtein modification; protein ubiquitination.
    • 配列類似性Belongs to the WD repeat CDC20/Fizzy family.
      Contains 7 WD repeats.
    • 発生段階Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
    • 翻訳後修飾Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
      Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C.
    • Information by UniProt
    • 参照データベース
    • 別名
      • bA276H19.3 antibody
      • Cdc 20 antibody
      • CDC20 antibody
      • CDC20 cell division cycle 20 homolog antibody
      • CDC20_HUMAN antibody
      • CDC20A antibody
      • Cell division cycle 20 antibody
      • Cell division cycle 20 homolog (S. cerevisiae) antibody
      • Cell division cycle 20 homolog antibody
      • Cell division cycle protein 20 homolog antibody
      • fizzy antibody
      • MGC102824 antibody
      • p55CDC antibody
      see all

    Anti-Cdc20 antibody 画像

    • ab86104, at 1/250 dilution, staining Cdc20 in formalin-fixed, paraffin-embedded human breast carcinoma tissue by Immunohistochemistry using DAB staining.
    • ab86104, at 1/250 dilution, staining Cdc20 in formalin-fixed, paraffin-embedded human colon carcinoma tissue by Immunohistochemistry using DAB staining.
    • ab86104, at 1/250 dilution, staining Cdc20 in formalin-fixed, paraffin-embedded mouse teratoma tissue by Immunohistochemistry using DAB staining.

    Anti-Cdc20 antibody (ab86104) 使用論文

    ab86104 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab86104.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"