Anti-Cdc20 抗体 (ab86104)
Key features and details
- Rabbit polyclonal to Cdc20
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cdc20 antibody
Cdc20 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cdc20 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide corresponding to a region between residues 50 and 100 of Human Cdc20 (NP_001246.2).
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ポジティブ・コントロール
- Human Anaplastic Thyroid Carcinoma, Breast Carcinoma, Colon Carcinoma and Prostate Carcinoma tissues. Mouse teratoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86104の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/500.
Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. |
特記事項 |
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IHC-P
1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. |
ターゲット情報
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機能
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the WD repeat CDC20/Fizzy family.
Contains 7 WD repeats. -
発生段階
Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition. -
翻訳後修飾
Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. - Information by UniProt
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参照データベース
- Entrez Gene: 991 Human
- Entrez Gene: 107995 Mouse
- Entrez Gene: 397379 Pig
- Omim: 603618 Human
- SwissProt: Q12834 Human
- SwissProt: Q9JJ66 Mouse
- SwissProt: Q5H7C0 Pig
- Unigene: 524947 Human
see all -
別名
- bA276H19.3 antibody
- Cdc 20 antibody
- CDC20 antibody
see all
画像
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ab86104, at 1/250 dilution, staining Cdc20 in formalin-fixed, paraffin-embedded human breast carcinoma tissue by Immunohistochemistry using DAB staining.
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ab86104, at 1/250 dilution, staining Cdc20 in formalin-fixed, paraffin-embedded human colon carcinoma tissue by Immunohistochemistry using DAB staining.
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ab86104, at 1/250 dilution, staining Cdc20 in formalin-fixed, paraffin-embedded mouse teratoma tissue by Immunohistochemistry using DAB staining.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab86104 は 3 報の論文で使用されています。
- Huo X et al. Identification of prognosis markers for endometrial cancer by integrated analysis of DNA methylation and RNA-Seq data. Sci Rep 9:9924 (2019). PubMed: 31289358
- Mitsui Y et al. CYP1B1 promotes tumorigenesis via altered expression of CDC20 and DAPK1 genes in renal cell carcinoma. BMC Cancer 15:942 (2015). ICC/IF . PubMed: 26626260
- Genga KR et al. Proteins of the mitotic checkpoint and spindle are related to chromosomal instability and unfavourable prognosis in patients with myelodysplastic syndrome. J Clin Pathol 68:381-7 (2015). PubMed: 25637637