製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Cdc20
  • アプリケーション
    適用あり: IHC-P, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 50 and 100 of human Cdc20 (NP_001246.2).

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
    • パスウェイ
      Protein modification; protein ubiquitination.
    • 配列類似性
      Belongs to the WD repeat CDC20/Fizzy family.
      Contains 7 WD repeats.
    • 発生段階
      Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
    • 翻訳後修飾
      Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
      Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C.
    • Information by UniProt
    • 参照データベース
    • 別名
      • bA276H19.3 antibody
      • Cdc 20 antibody
      • CDC20 antibody
      • CDC20 cell division cycle 20 homolog antibody
      • CDC20_HUMAN antibody
      • CDC20A antibody
      • Cell division cycle 20 antibody
      • Cell division cycle 20 homolog (S. cerevisiae) antibody
      • Cell division cycle 20 homolog antibody
      • Cell division cycle protein 20 homolog antibody
      • fizzy antibody
      • MGC102824 antibody
      • p55CDC antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Cdc20 with ab72202 at 1/1000 (1µg/ml). Detection: DAB.

    参考文献

    ab72202 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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