製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Cdc20
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human) from an internal sequence of Cdc20.

  • ポジティブ・コントロール
    • HeLa and Jurkat cell extracts.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab64877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ELISA 1/20000.

ターゲット情報

  • 機能
    Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Belongs to the WD repeat CDC20/Fizzy family.
    Contains 7 WD repeats.
  • 発生段階
    Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
  • 翻訳後修飾
    Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
    Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA276H19.3 antibody
    • Cdc 20 antibody
    • CDC20 antibody
    • CDC20 cell division cycle 20 homolog antibody
    • CDC20_HUMAN antibody
    • CDC20A antibody
    • Cell division cycle 20 antibody
    • Cell division cycle 20 homolog (S. cerevisiae) antibody
    • Cell division cycle 20 homolog antibody
    • Cell division cycle protein 20 homolog antibody
    • fizzy antibody
    • MGC102824 antibody
    • p55CDC antibody
    see all

画像

  • All lanes : Anti-Cdc20 antibody (ab64877) at 1/500 dilution

    Lane 1 : extract from HeLa cells, (5-30µg total protein).
    Lane 2 : extract from Jurkat cells, (5-30µg total protein).
    Lane 3 : extract from Jurkat cells, (5-30µg total protein) and 5-10µg of the immunising peptide.


    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 26 kDa,34 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

This product has been referenced in:
  • Tan CL  et al. Stranglehold on the spindle assembly checkpoint: the human papillomavirus E2 protein provokes BUBR1-dependent aneuploidy. Cell Cycle 14:1459-70 (2015). WB ; Human . Read more (PubMed: 25789401) »
  • Adhikari D  et al. Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II. J Cell Biol 206:843-53 (2014). Mouse . Read more (PubMed: 25246615) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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