Anti-Cdc13 抗体 [6F11/2] (ab10873)
Key features and details
- Mouse monoclonal [6F11/2] to Cdc13
- Suitable for: WB
- Isotype: IgG2a
製品の概要
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製品名
Anti-Cdc13 antibody [6F11/2] -
製品の詳細
Mouse monoclonal [6F11/2] to Cdc13 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差が予測される動物種: Schizosaccharomyces pombe -
免疫原
Full length native protein (purified).
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特記事項
Cdc13 is a fission yeast (S. pombe) B-type M-phase cyclin. Cdc13 binds to Cdk1 (cdc2), and the resulting Cdk1-Cdc13 complex controls the G2/M transition of the cell cycle. 6F11/2 can be used for detecting Cdc13 and associated Cdk1 kinase activation.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A purified -
一次抗体 備考
Cdc13 is a fission yeast (S. pombe) B-type M-phase cyclin. Cdc13 binds to Cdk1 (cdc2), and the resulting Cdk1-Cdc13 complex controls the G2/M transition of the cell cycle. 6F11/2 can be used for detecting Cdc13 and associated Cdk1 kinase activation. -
ポリ/モノ
モノクローナル -
クローン名
6F11/2 -
ミエローマ
Sp2 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10873の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 48 kDa. |
ターゲット情報
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参照データベース
- Entrez Gene: 2540880 Schizosaccharomyces pombe
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別名
- CDC 13 antibody
- G2/mitotic specific cyclin antibody
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab10873 は 7 報の論文で使用されています。
- Basu S et al. Transcription factor Atf1-dependent degradation of the mitotic cyclin Cdc13 is regulated by multiple factors in Schizosaccharomyces pombe. FEBS Lett 596:2021-2030 (2022). PubMed: 35770329
- Basu S et al. The Hydrophobic Patch Directs Cyclin B to Centrosomes to Promote Global CDK Phosphorylation at Mitosis. Curr Biol 30:883-892.e4 (2020). PubMed: 32084401
- Rothe C et al. A checkpoint-independent mechanism delays entry into mitosis after UV irradiation. J Cell Sci 130:4028-4037 (2017). PubMed: 29046339
- Uchiyama M et al. The Protein Level of Rev1, a TLS Polymerase in Fission Yeast, Is Strictly Regulated during the Cell Cycle and after DNA Damage. PLoS One 10:e0130000 (2015). PubMed: 26147350
- Bandyopadhyay S et al. The basic leucine zipper domain transcription factor Atf1 directly controls Cdc13 expression and regulates mitotic entry independently of Wee1 and Cdc25 in Schizosaccharomyces pombe. Eukaryot Cell 13:813-21 (2014). Schizosaccharomyces pombe . PubMed: 24728197
- Okuzaki D et al. Spo5 phosphorylation is essential for its own timely degradation and for successful meiosis in Schizosaccharomyces pombe. Cell Cycle 9: (2010). WB ; Schizosaccharomyces pombe . PubMed: 20855961
- Decottignies A et al. In vivo localisation of fission yeast cyclin-dependent kinase cdc2p and cyclin B cdc13p during mitosis and meiosis. J Cell Sci 114:2627-40 (2001). PubMed: 11683390