製品の概要

  • 製品名Anti-CD99R antibody [MEM-131] (FITC)
    CD99R 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-131] to CD99R (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    T cell line HPB-ALL. (Human)

  • 特記事項

    The antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab26007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 5-20µl for 106 cells. ab91546-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes.
  • 配列類似性Belongs to the CD99 family.
  • 翻訳後修飾Extensively O-glycosylated.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1110061M03Rik antibody
    • 12E7 antibody
    • 2410026K10Rik antibody
    • antigen identified by monoclonal 12E7, Y homolog antibody
    • antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
    • CD99 antibody
    • CD99 antigen antibody
    • CD99 antigen, X chromosome antibody
    • CD99 molecule antibody
    • CD99_HUMAN antibody
    • Cell surface antigen 12E7, X chromosome antibody
    • Cell surface antigen HBA-71, X chromosome antibody
    • Cell surface antigen O13, X chromosome antibody
    • D4 antibody
    • E2 antigen antibody
    • E2 antigen, X chromosome antibody
    • HBA71 antibody
    • MIC2 (monoclonal antibody 12E7) antibody
    • MIC2 antibody
    • MIC2 surface antigen, X chromosome antibody
    • MIC2X antibody
    • MIC2Y antibody
    • MSK5X antibody
    • Paired immunoglobin-like type 2 receptor-ligand antibody
    • pilr-1 antibody
    • Pilr-l antibody
    • Protein MIC2 antibody
    • T cell surface glycoprotein E2 antibody
    • T-cell surface glycoprotein E2 antibody
    see all

Anti-CD99R antibody [MEM-131] (FITC) 画像

  • Overlay histogram showing Jurkat cells stained with ab26007 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26007, 5µl/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (FITC) (ab91546, 2µg/1x106). Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Jurkat cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-CD99R antibody [MEM-131] (FITC) (ab26007) 使用論文

This product has been referenced in:
  • Cermak L  et al. Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules. J Biol Chem 277:7955-61 (2002). Read more (PubMed: 11773067) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"