製品の概要

  • 製品名Anti-CD99 antibody [EPR3096]
    CD99 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3096] to CD99
  • アプリケーション適用あり: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to the extracellular domains in Human CD99.

  • ポジティブ・コントロール
    • THP-1, Jurkat, U937, Molt-4, HuT-78, and HUVEC cell lysates, Human pancreas, Human tonsil and Human uterus tissues
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-CD99 antibody (Alexa Fluor® 488) [EPR3096] (ab208372)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 28, 32 kDa (predicted molecular weight: 19 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.
  • 配列類似性Belongs to the CD99 family.
  • 翻訳後修飾Extensively O-glycosylated.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 12E7 antibody
    • Antigen identified by monoclonal 12E7, Y homolog antibody
    • Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
    • CD99 antibody
    • CD99 antigen antibody
    • CD99 molecule antibody
    • CD99_HUMAN antibody
    • Cell surface antigen 12E7 antibody
    • Cell surface antigen HBA 71 antibody
    • Cell surface antigen O13 antibody
    • E2 antigen antibody
    • HBA71 antibody
    • MIC 2X antibody
    • MIC 2Y antibody
    • MIC2 (monoclonal antibody 12E7) antibody
    • MIC2 antibody
    • MIC2X antibody
    • MIC2Y antibody
    • MSK5X antibody
    • Protein MIC2 antibody
    • Surface antigen MIC2 antibody
    • T cell surface glycoprotein E2 antibody
    • T-cell surface glycoprotein E2 antibody
    see all

Anti-CD99 antibody [EPR3096] 画像

  • Immunohistochemical staining of paraffin-embedded Human pancreas tissue using ab108297 at a dilution of 1/250.
  • All lanes : Anti-CD99 antibody [EPR3096] (ab108297) at 1/1000 dilution

    Lane 1 : THP-1 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : U937 cell lysate
    Lane 4 : Molt-4 cell lysate
    Lane 5 : HuT-78 cell lysate
    Lane 6 : HUVEC cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 19 kDa
    Observed band size : 28,32 kDa (why is the actual band size different from the predicted?)
  •  Flow cytometric analysis of Molt-4 cells using anti-CD99 RabMAb (red) (ab108297) or a rabbit IgG (negative) (green).

  • Fluorescent immunohistochemical analysis of paraffin-embedded human normal pancreas tissue using ab108297. Green-CD99 red-PI

  • Immunohistochemical staining of paraffin-embedded Human tonsil tissue using ab108297 at a dilution of 1/250.
  • Immunohistochemical staining of paraffin-embedded Human uterus tissue using ab108297 at a dilution of 1/250.

Anti-CD99 antibody [EPR3096] (ab108297) 使用論文

This product has been referenced in:
  • Weekes MP  et al. Quantitative temporal viromics: an approach to investigate host-pathogen interaction. Cell 157:1460-72 (2014). WB ; Human . Read more (PubMed: 24906157) »

See 1 Publication for this product

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Thank you for your enquiry and your interest in our products.

Each antibody should be titrated to determine the optimal working dilutions. It is the customer's responsibility to calculate how much product is needed for each assay.

T...

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Thank you for contacting Abcam.

We currently do not have any product against the C.elegans protein MKS-5 nor will our products against a similar proteinRPGRIP1L recognize MKS-5. A protein with a similar name is MSK5X (also known as CD99). We...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"