The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報IHC-P: 1/200 for 10 min at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM Citrate buffer pH 6.0 for 10 min and then cool at room temperature for 20 min.
Staining pattern: Cell membrane
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 19 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.
Cooper A et al. Ewing tumors that do not overexpress BMI-1 are a distinct molecular subclass with variant biology: a report from the Children's Oncology Group. Clin Cancer Res17:56-66 (2011).
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Luo W et al. GSTM4 is a microsatellite-containing EWS/FLI target involved in Ewing's sarcoma oncogenesis and therapeutic resistance. Oncogene28:4126-32 (2009).
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