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The details of the immunogen for this antibody are not available.
ab24904 stimulates T-cell proliferation and IL2 secretion, via signalling through the T-cell receptor/CD3 complex. ab24904 has also been reported to promote apoptosis of thymocytes and CTL clones and to mediate adhesion of thymocytes to thymic stroma.
Our Abpromise guarantee covers the use of ab24904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.
Induction of apoptosis.
|Flow Cyt||Use 2µg for 106 cells.
ab154450 - Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|Cellular Activation||Use at an assay dependent concentration.|
Flow cytometry analysis staining CD90 / Thy1 in mouse splenocytes using ab24904 at a dilution of 0.03 μg/106 cells.