Anti-CD90 / Thy1 抗体 [F15-42-1] (FITC) (ab11155)

Submit an Abreview Q&A (6)Specific References (8)

製品の概要

  • 製品名
    Anti-CD90 / Thy1 antibody [F15-42-1] (FITC)
    CD90 / Thy1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [F15-42-1] to CD90 / Thy1 (FITC)
  • 由来種
    Mouse
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • アプリケーション
    適用あり: Flow Cytmore details
  • 種交差性
    交差種: Human, Monkey
  • 免疫原

    Synthetic peptide corresponding to Human CD90/ Thy1.

  • ポジティブ･コントロール
    • MOLT4 human T-cell lines. human T lymphoma cell line HUT78.
  • 特記事項
    This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

Use neat. Use 10µl of the suggested working dilution to label 106 cells in 100µl.

 

 

 

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 機能
    May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • 配列類似性
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • CD7 antibody
      • CD90 antibody
      • CD90 antigen antibody
      • CDw90 antibody
      • FLJ33325 antibody
      • MGC128895 antibody
      • T25 antibody
      • Theta antigen antibody
      • Thy 1 antibody
      • Thy 1 cell surface antigen antibody
      • Thy 1 membrane glycoprotein antibody
      • Thy 1 T cell antigen antibody
      • Thy 1.2 antibody
      • Thy-1 antigen antibody
      • Thy-1 membrane glycoprotein antibody
      • Thy1 antibody
      • Thy1 antigen antibody
      • Thy1 T cell antigen antibody
      • Thy1.1 antibody
      • Thy1.2 antibody
      • THY1_HUMAN antibody
      • Thymus cell antigen 1, theta antibody
      see all

    画像

    • Flow Cytometry - Anti-CD90 / Thy1 antibody [F15-42-1] (FITC) (ab11155)
      Flow Cytometry - Anti-CD90 / Thy1 antibody [F15-42-1] (FITC) (ab11155)
      ab11155 staining CD90/Thy1 in human HUT78 T cells by Flow Cytometry analysis.

    プロトコール

    参考文献

    This product has been referenced in:
    • S Lashkari B & Anumba DO Estradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4. PLoS One 12:e0173646 (2017). Read more (PubMed: 28296959) »
    • Shi Q  et al. Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into endometrial cells. Stem Cell Res Ther 8:246 (2017). Read more (PubMed: 29096715) »

    See all 8 Publications for this product

    ab11155 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    6 reviews or Q&A

    10
    Votes: Highest First

    May I know if the following antibodies can be used for ICC and/or western blot? 1) CD31 (ab27333) 2) CD71 (ab47095) 3) CD44 (ab30437) 4) CD90 (ab11155) Also, can the IgG1(ab106163) be used as the isotype control for FACS with the 4 antibodies listed ab...

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    Thank you very much for your interest in abcam antibodies. To our knowledge,these primary antibodies (ab27333, ab47095, ab30437 and ab11155)have not been tested in Western Blot and ICC/IF. They have been tested only in Flow cytometry as shown on the da...

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    Sorry for forgetting to ask another question. I noted that the application of some antibodies is only for flow cytometry.CouldI use these antibodies to do western blot orICC? Thank you!

    If the antibody datasheets only list "flow cytometry" in the Applications section, then that is the only application that has been tested. It is possible that the antibodies could also work for western blotting or ICC, but these applications ...

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    Thanks so much for your kind help. As I known, for flow cytometry, people always use normal cell(without antibody disposal) as control to set up the machine before running. But there are also some people use isotype antibody to replace normal cell as c...

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    A cells-only control with no antibody will show the contribution of autofluorescence of the cells themselves. Most people use this to set up the flow cytometer. An isotype control (cells incubated with the same isotype of antibody as the target-specifi...

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    since these antibodies I choose are FITC-conjugated, need I use F4/1(ab112511) as anti-FITC antibody for flowcytomety set? Hope to hear from you soon. Many thanks!

    Thank you for contacting Abcam. I am replying to your question on behalf of my colleague Ada. The antibodies that have FITC conjugated to them do not need the anti-FITC antibody ab112511, if you simply want to detect FITC fluorescence by flow cytometry...

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    Hi,   Our customer would like to know if the following antibodies are of IgG1, kappa  or lambda light chains (Information on datasheet only stated Isotypes IgG1/ IgM/ IgG2a)?   Item                                      Format                 Cat # Anti...

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    Thank you for contacting us. In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not be...

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    The customer would like to know if the antibody will label both fibroblasts and/or smooth muscle cells, if it labels both do we know how abundant is CD90 on smooth muscle cells?

    Thank you for your enquiry. We test this antibody on the human T lymphoma cell line HUT78 but CD90 is reportedly expressed by fibroblast subsets. The following reference may be of interest, it uses this clone (F15-42-1) for staining of fibroblast...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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