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Our Abpromise guarantee covers the use of ab23894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ELISA||Use at an assay dependent concentration.|
ab23894 staining CD90 / Thy1 in Limbal biopsies from Human corneas by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde, permeabilized with 0.5% Triton and blocked with 10% goat serum in PBS for 90 minutes at room temperature. Samples were incubated with primary antibody overnight at 4°C. An Alexa Fluor® 594-conjugated Goat anti-mouse polyclonal (1/250) was used as the secondary antibody.
CD90 expression is markedly increased by limbal stromal cells underlying the limbal crypts (E) compared to the non-crypt rich limbus (C). Central corneal sections were used as a negative control (A). Sections were counterstainded with DAPI (blue). Scale bars: 50um.
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