製品の概要

  • 製品名Anti-CD9 antibody [MEM-61]
    CD9 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-61] to CD9
  • 特異性This antibody reacts with human CD9 antigen.
  • アプリケーション適用あり: WB, IP, IHC-P, Flow Cyt, Blocking, Functional Studiesmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Tissue/ cell preparation (Human). (Pre-B line Nalm-6).

  • ポジティブ・コントロール
    • Prostate IHC-P: Human lung FFPE tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 - 4 µg/ml. Use under non reducing condition.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 20 µg/ml.
Flow Cyt Use a concentration of 1 - 5 µg/ml.
Blocking Use at an assay dependent concentration. PubMed: 22745469
Functional Studies Use at an assay dependent concentration. The antibody induces FcgR-dependent platelet activation (aggregation).

ターゲット情報

  • 機能Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
  • 組織特異性Expressed by a variety of hematopoietic and epithelial cells.
  • 配列類似性Belongs to the tetraspanin (TM4SF) family.
  • 翻訳後修飾Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Tetraspanin 29 antibody
    • 5H9 antibody
    • 5H9 antigen antibody
    • Antigen defined by monoclonal antibody 602 29 antibody
    • Antigen defined by monoclonal antibody 60229 antibody
    • BA-2/p24 antigen antibody
    • BA2 antibody
    • BTCC 1 antibody
    • BTCC1 antibody
    • CD9 antibody
    • CD9 antigen antibody
    • CD9 antigen p24 antibody
    • CD9 molecule antibody
    • CD9_HUMAN antibody
    • Cell growth inhibiting gene 2 protein antibody
    • Cell growth-inhibiting gene 2 protein antibody
    • DRAP 27 antibody
    • DRAP27 antibody
    • GIG2 antibody
    • Growth inhibiting gene 2 protein antibody
    • Leukocyte antigen MIC3 antibody
    • MIC3 antibody
    • Motility related protein antibody
    • Motility-related protein antibody
    • MRP 1 antibody
    • MRP-1 antibody
    • MRP1 antibody
    • p24 antibody
    • p24 antigen antibody
    • Tetraspanin-29 antibody
    • Tspan 29 antibody
    • Tspan-29 antibody
    • TSPAN29 antibody
    see all

Anti-CD9 antibody [MEM-61] 画像

  • IHC image of ab2215 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2215, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing Jurkat cells stained with ab2215 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2215, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-CD9 antibody [MEM-61] (ab2215) 使用論文

This product has been referenced in:
  • Salomon C  et al. A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration. PLoS One 9:e98667 (2014). WB ; Human . Read more (PubMed: 24905832) »
  • Rappa G  et al. The nuclear pool of tetraspanin CD9 contributes to mitotic processes in human breast carcinoma. Mol Cancer Res 12:1840-50 (2014). Read more (PubMed: 25103498) »

See all 6 Publications for this product

Product Wall

ab2215 should be used at a concentration of 2-4 ug/ml in blocking buffer when incubating your membrane whereas ab8216 should be used at a concentration of 2 ug/ml.

The gel should be run in non-reducing conditions as these antibodies do not re...

Read More

Thank you for contacting us.

The antibodies have not been tested for reactivity with bovine samples, and we have not received reports of bovine reactivity from our customers.

Please do not hesitate to contact us if you need any mo...

Read More

I'm very sorry to hear you are experiencing a problem with ab2215. I appreciate the urgency in resolving this problem due to the short time you gave in the laboratory, and I believe that with the following protocol tips you will have improved results...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"