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Tissue/ cell preparation (Human). (Pre-B line Nalm-6).
Our Abpromise guarantee covers the use of ab2215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 - 4 µg/ml. Use under non reducing condition.|
|IHC-P||Use a concentration of 20 µg/ml.|
|Flow Cyt||Use a concentration of 5 µg/ml.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|Blocking||Use at an assay dependent concentration. PubMed: 22745469|
|Functional Studies||Use at an assay dependent concentration. The antibody induces FcgR-dependent platelet activation (aggregation).|
IHC image of ab2215 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2215, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.