製品の概要

  • 製品名Anti-CD9 antibody [EM-04]
    CD9 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [EM-04] to CD9
  • アプリケーション適用あり: WB, IP, ICC, Flow Cytmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Tissue/ cell preparation of permeabilized murine bone marrow-derived mast cells (BMMC)

  • ポジティブ・コントロール
    • Lysates from Mouse bone marrow-derived mast cells (BMMC)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82390 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Use under non reducing condition. Predicted molecular weight: 25 kDa.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use a concentration of 4 µg/ml. ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
  • 組織特異性Expressed by a variety of hematopoietic and epithelial cells.
  • 配列類似性Belongs to the tetraspanin (TM4SF) family.
  • 翻訳後修飾Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Tetraspanin 29 antibody
    • 5H9 antibody
    • 5H9 antigen antibody
    • Antigen defined by monoclonal antibody 602 29 antibody
    • Antigen defined by monoclonal antibody 60229 antibody
    • BA-2/p24 antigen antibody
    • BA2 antibody
    • BTCC 1 antibody
    • BTCC1 antibody
    • CD9 antibody
    • CD9 antigen antibody
    • CD9 antigen p24 antibody
    • CD9 molecule antibody
    • CD9_HUMAN antibody
    • Cell growth inhibiting gene 2 protein antibody
    • Cell growth-inhibiting gene 2 protein antibody
    • DRAP 27 antibody
    • DRAP27 antibody
    • GIG2 antibody
    • Growth inhibiting gene 2 protein antibody
    • Leukocyte antigen MIC3 antibody
    • MIC3 antibody
    • Motility related protein antibody
    • Motility-related protein antibody
    • MRP 1 antibody
    • MRP-1 antibody
    • MRP1 antibody
    • p24 antibody
    • p24 antigen antibody
    • Tetraspanin-29 antibody
    • Tspan 29 antibody
    • Tspan-29 antibody
    • TSPAN29 antibody
    see all

Anti-CD9 antibody [EM-04] 画像

  • All lanes : Anti-CD9 antibody [EM-04] (ab82390) at 1 µg/ml

    Lane 1 : lysate from 1X106 bone marrow-derived mast cell (BMMC)
    Lane 2 : lysate from 0.5X106 bone marrow-derived mast cell (BMMC)
    Lane 3 : lysate from 0.25X106 bone marrow-derived mast cell (BMMC)
    Lane 4 : lysate from 0.125X106 bone marrow-derived mast cell (BMMC)


    Performed under non-reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

Anti-CD9 antibody [EM-04] (ab82390) 使用論文

ab82390 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Human Cell (Blood film)
Specification Blood film
Permeabilization Yes - 0.1M PBS with 1% TritonX100
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Sep 26 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"