Anti-CD89 抗体 [EPR4622(2)] (ab124717)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4622(2)] to CD89
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD89 antibody [EPR4622(2)]
CD89 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4622(2)] to CD89 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details
適用なし: Flow Cyt (Intra) -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human CD89 aa 250-350 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: U937, K562, Jurkat, and HL-60 cell lysates; IHC: Human tonsil tissue; IP: U-937.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4622(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124717の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Binds to the Fc region of immunoglobulins alpha. Mediates several functions including cytokine production. -
組織特異性
Isoform A.1, isoform A.2 and isoform A.3 are differentially expressed between blood and mucosal myeloid cells. Isoform A.1, isoform A.2 and isoform A.3 are expressed in monocytes. Isoform A.1 and isoform A.2 are expressed in alveolar macrophages; however only one isoform is expressed at alveolar macrophages surfaces. -
配列類似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2204 Human
- Omim: 147045 Human
- SwissProt: P24071 Human
- Unigene: 659872 Human
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別名
- CD89 antibody
- Fc alpha receptor antibody
- Fc fragment of IgA, receptor for antibody
see all
画像
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ab124717, at 1/100, staining CD89 in paraffin embedded Human tonsil tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-CD89 antibody [EPR4622(2)] (ab124717) at 1/1000 dilution
Lane 1 : U937 cell lysates
Lane 2 : K562 cell lysates
Lane 3 : Jurkat cell lysates
Lane 4 : HL-60 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 32 kDa -
CD89 was immunoprecipitated from 0.35 mg U-937 (Human histiocytic lymphoma monocyte) whole cell lysate 10 µg with 124717 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: U-937 (Human histiocytic lymphoma monocyte) whole cell lysate 10 µg
Lane 2: ab124717 IP in U-937 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab124717 in U-937 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab124717 は 2 報の論文で使用されています。
- Cho YT et al. Clinical, Histopathologic, and Immunohistochemical Features of Patients with IgG/IgA Pemphigus. Biomedicines 10:N/A (2022). PubMed: 35625932
- Yuan FY et al. Bone marrow stromal cells-derived exosomes target DAB2IP to induce microglial cell autophagy, a new strategy for neural stem cell transplantation in brain injury. Exp Ther Med 20:2752-2764 (2020). PubMed: 32765770