製品の概要

  • 製品名
    Anti-CD86 antibody [GL1] (PE/Cy7 ®)
    CD86 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [GL1] to CD86 (PE/Cy7 ®)
  • 標識
    PE/Cy7 ®. Ex: 496nm, Em: 774nm
  • アプリケーション
    適用あり: Flow Cytmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    LPS activated mouse B cells

  • 特記事項
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.

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ターゲット情報

  • 機能
    Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
  • 組織特異性
    Expressed by activated B-lymphocytes and monocytes.
  • 配列類似性
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻訳後修飾
    Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activation B7-2 antigen 3 antibody
    • Activation B7-2 antigen antibody
    • B-lymphocyte activation antigen B7-2 2 antibody
    • B-lymphocyte activation antigen B7-2 antibody
    • B7 2 antibody
    • B7 antibody
    • B7-2 antibody
    • B7.2 antibody
    • B70 antibody
    • B72 antigen antibody
    • BU63 antibody
    • CD28 antigen ligand 2 2 antibody
    • CD28 antigen ligand 2 antibody
    • Cd28l2 antibody
    • CD28LG2 antibody
    • CD86 antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) antibody
    • CD86 antigen antibody
    • CD86 molecule antibody
    • CD86_HUMAN antibody
    • CLS1 antibody
    • CTLA-4 counter-receptor B7.2 2, 3 antibody
    • CTLA-4 counter-receptor B7.2 antibody
    • Early T-cell costimulatory molecule 1 antibody
    • ETC-1 antibody
    • FUN 1 antibody
    • FUN-1 antibody
    • LAB72 antibody
    • Ly-58 antibody
    • Ly58 antibody
    • MB7 antibody
    • MB7-2 antibody
    • Membrane glycoprotein antibody
    • MGC34413 antibody
    • T-lymphocyte activation antigen CD86 antibody
    • TS/A-2 antibody
    see all

プロトコール

参考文献

This product has been referenced in:
  • Hathcock KS  et al. Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function. J Exp Med 180:631-40 (1994). Read more (PubMed: 7519245) »
  • Inaba K  et al. The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. J Exp Med 180:1849-60 (1994). Read more (PubMed: 7525841) »

See all 4 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (Spleen cell)
Permeabilization
No
Gating Strategy
CD45+ CD11c+ CD86+
Specification
Spleen cell
Preparation
Cell harvesting/tissue preparation method: Smush spleens through 70um filter, then lyse RBCs
Sample buffer: PBS + 2% FBS
Fixation
Formaldehyde
Username

Ms. Cheryl Sandoval

Verified customer

投稿 Jun 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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