Anti-CD82 抗体 [B-L2] - BSA and Azide free (ab47153)
Key features and details
- Mouse monoclonal [B-L2] to CD82 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CD82 antibody [B-L2] - BSA and Azide free
CD82 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [B-L2] to CD82 - BSA and Azide free -
由来種
Mouse -
特異性
Recognises the CD82 antigen, a 60 kDa protein. Cross reacts with mouse CD82 protein in cytometry.
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アプリケーション
適用あり: IHC-P, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CD82. CD82 transfected P815 cell line
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Ion Exchange Chromatography -
特記事項(精製)
Sterile-filtered through 0.22 µm and treated to remove endotoxins. Carrier and preservative free. -
ポリ/モノ
モノクローナル -
クローン名
B-L2 -
ミエローマ
x63-Ag8.653 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47153の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway. -
組織特異性
Lymphoid specific. -
配列類似性
Belongs to the tetraspanin (TM4SF) family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3732 Human
- Omim: 600623 Human
- SwissProt: P27701 Human
- Unigene: 527778 Human
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別名
- 4F9 antibody
- Antigen detected by monoclonal and antibody IA4 antibody
- C33 antibody
see all
画像
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A typical staining pattern with the B-L2 monoclonal antibody of lymphocytes
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IHC image of ab47153 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47153, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab47153 は 3 報の論文で使用されています。
- Di Giacomo V et al. ?Np63a promotes adhesion of metastatic prostate cancer cells to the bone through regulation of CD82. Oncogene 36:4381-4392 (2017). Flow Cyt . PubMed: 28368419
- Marjon KD et al. Tetraspanin CD82 regulates bone marrow homing of acute myeloid leukemia by modulating the molecular organization of N-cadherin. Oncogene 35:4132-40 (2016). ICC/IF . PubMed: 26592446
- Artavanis-Tsakonas K et al. The Tetraspanin CD82 is Specifically Recruited to Fungal and Bacterial Phagosomes Prior to Acidification. Infect Immun : (2010). ICC/IF ; Human . PubMed: 21149584