製品の概要

  • 製品名
    Anti-CD8 antibody [YTS169.4]
    CD8 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [YTS169.4] to CD8
  • アプリケーション
    適用あり: ICC/IF, Flow Cyt, IHC-Frmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    CBA mouse spleen cells and thymocytes

  • ポジティブ・コントロール
    • Flow Cytometry: Mouse Spleen Cells
  • 特記事項

    ab22378 is not expected to work in standard western blots, under denaturing conditions, or in IHC on formalin-fixed tissue. To our knowledge, ab22378 will only stain frozen sections or fresh cells, and will only immunoprecipitate the antigen from cell lysates in mild detergents such as NP40 or Triton-X100.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab22378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 0.1-1µg for 106 cells.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • 関連疾患
    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • 配列類似性
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻訳後修飾
    All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
  • 別名
    • CD8 antibody
    • CD8 antigen, alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8a antigen antibody
    • CD8a molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    see all

画像

  • ab22378 staining CD8 in murine bone marrow cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed prior to blocking in 2% BSA  for 30 minutes at 20°C. The primary antibody (1/200) was incubated with the sample for 9 hours at 4°C. An undiluted Alexa Fluor® 555-conjugated goat anti-rat polyclonal was used as the secondary. The nuclei were stained with DAPI.

    See Abreview

  • ab22378 staining CD8 in mouse spleen tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 2 hours at 25°C. The sample was incubated with primary antibody (1/250 in PBS-Tween) at 4°C for 12 hours. An Alexa Fluor® 594-conjugated Goat polyclonal to rat IgG (1/250) was used as secondary antibody. Nuclei were stained with DAPI.

    See Abreview

  • Overlay histogram showing mouse spleen cells (C57BL/6) stained with ab22378 (red line). The cells were incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22378, 0.1μg/1x10^6 cells) for 30 min at 4ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rat IgG (H&L) (ab150157) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 2μg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Normal mouse (CBA) spleen cells (red cell lysed) double stained with ab22378 (0.3 µg/mL, detected with Alexa Fluor® 488-conjugated monoclonal mouse anti-rat antibody) and an APC conjugated hamster anti-mouse CD3 antibody. ab22378 stains an expected subpopulation of CD8 alpha positive T cells.

参考文献

This product has been referenced in:
  • He J  et al. The influence of exendin-4 intervention on -obese diabetic mouse blood and the pancreatic tissue immune microenvironment. Exp Ther Med 12:2893-2898 (2016). Flow Cyt ; Mouse . Read more (PubMed: 27882092) »
  • Danielson LS  et al. Limited miR-17-92 overexpression drives hematologic malignancies. Leuk Res 39:335-41 (2015). IHC ; Mouse . Read more (PubMed: 25597017) »

See all 21 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (tumor tissue)
Permeabilization
Yes - 5% Triton X-100 in PBS
Specification
tumor tissue
Blocking step
Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 27 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (MC38 tumor)
Permeabilization
Yes - 1x PBS with 0.3% triton-x-100
Specification
MC38 tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Formaldehyde
Username

Dr. Ravikumar Muthuswamy

Verified customer

投稿 May 11 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Mouse Tissue sections (skin wound)
Specification
skin wound
Permeabilization
No
Fixative
Acetone
Username

Abcam user community

Verified customer

投稿 Mar 28 2014

Thank you for your email.

There may be some antibodies where I might have suggested the human tissues or cells. I apologize for this. I have included the positive control information irrespective of species so similar mouse tissues sections ca...

Read More

Thank you for contacting us.

The positive control for ab36392 is IL2 activatedcultured NK cells.

ab9918; human tonsil sections, human cervical squamous cell carcinoma section

ab34674; human artery or colon tissue sections
Read More

Thank you for contacting us.

We suggest to optimise the working concentration according to the sample. Initially, you could try using 1:250 for ab22378 and 2ug/ml for ab119857.

I hope this information is helpful to you. Please do ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Lymph Node)
Specification
Lymph Node
Fixative
Acetone/Alcohol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Mar 19 2012

Thank you for your query.
Unfortunately we have not mapped this clone and we currently do not have any empirical data to indicate which region of CD8 is recognized.
However, online papers mentioning the clone indicate it recognizes a band of ...

Read More

Thank you for your inquiry ab85792. Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information. We aim to provide as much information as possible to our customers, so I am sorry that ...

Read More

Thank you for contacting us. Your credit note ID is XXXX. I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the fo...

Read More

1-10 of 20 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録