製品の概要

  • 製品名Anti-CD8 antibody [MEM-31] (PerCP)
    CD8 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MEM-31] to CD8 (PerCP)
  • 標識PerCP. Ex: 482nm, Em: 675nm
  • 特異性ab65949 recognizes a conformationally-dependent epitope of CD8. The antibody does not react with formaldehyde-fixed cells and is negative in Western Blotting.
  • アプリケーション適用あり: Flow Cytmore details
    適用なし: WB
  • 種交差性
    交差種: Human
  • 免疫原

    Tissue/ cell preparation (Human):Crude thymus membrane fraction.

  • ポジティブ・コントロール
    • human blood
  • 特記事項

    Conjugated with Peridinin-chlorophyll-protein complex (PerCP)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
  • 追加情報Flow Cyt: Use Neat: 10µl for 106 cells, or 100µl whole blood.


    Is unsuitable for WB.
    Is unsuitable for applications using formaldehyde fixed cells.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
    • 関連疾患Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
    • 配列類似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • 翻訳後修飾All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
    • 細胞内局在Secreted and Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 製品の状態CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
    • 別名
      • CD8 antibody
      • CD8 antigen, alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8a antigen antibody
      • CD8a molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      see all

    Anti-CD8 antibody [MEM-31] (PerCP) 画像

    • ab65949 staining CD86 in Human whole blood by Flow Cytometry. The sample was incubated with the primary antibody (1/50 in 2% Human serum + 0.5mM EDTA in PBS) for 25 minutes at 4°C.
      Gating Strategy: Lymphocytes.

       

      See Abreview

    Anti-CD8 antibody [MEM-31] (PerCP) (ab65949) 使用論文

    This product has been referenced in:
    • Linnebacher M  et al. Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO). J Biomed Biotechnol 2010:841451 (2010). Read more (PubMed: 20339516) »

    See 1 Publication for this product

    Product Wall

    Application Flow Cytometry
    Permeabilization No
    Sample Human Cell (whole blood)
    Specification whole blood
    Gating Strategy lymphocytes
    Preparation Cell harvesting/tissue preparation method: Ficoll-Hypaque
    Sample buffer: PBS
    Username

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    投稿 Nov 19 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"