Anti-CD8 alpha 抗体 [EP1150Y] (ab93278)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1150Y] to CD8 alpha
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD8 alpha antibody [EP1150Y]
CD8 alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1150Y] to CD8 alpha -
由来種
Rabbit -
特異性
The immunogen sequence for this antibody has no significant homology with mouse and rat CD alpha and beta. This product showed specific staining in lymphocytes, although we observed background staining in some epithelial cells tested. -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: IHC-P -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human CD8 alpha aa 200 to the C-terminus (C terminal).
Database link: P01732 -
ポジティブ・コントロール
- WB: THP-1 cell lysate. ICC/IF: MOLT-4 cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1150Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93278の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Predicted molecular weight: 26 kDa.
For unpurified use at 1/1000 - 1/10000. |
|
ICC/IF |
1/500.
For unpurified use at 1/50 - 1/100. |
特記事項 |
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WB
1/1000. Predicted molecular weight: 26 kDa. For unpurified use at 1/1000 - 1/10000. |
ICC/IF
1/500. For unpurified use at 1/50 - 1/100. |
ターゲット情報
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機能
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
関連疾患
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 925 Human
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
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別名
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
画像
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Immunocytochemistry analysis of MOLT-4 (human lymphoblastic leukemia T lymphoblast) labeling CD8 alpha with purified ab93278 at 1/250 dilution (9 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.21 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody. -
Anti-CD8 alpha antibody [EP1150Y] (ab93278) at 1/1000 dilution (unpurified) + THP-1 cell lysate at 10 µg
Predicted band size: 26 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (45)
ab93278 は 45 報の論文で使用されています。
- Liu J et al. Multicenter phase II trial of Camrelizumab combined with Apatinib and Eribulin in heavily pretreated patients with advanced triple-negative breast cancer. Nat Commun 13:3011 (2022). PubMed: 35641481
- Lei X et al. Mitochondrial fission induces immunoescape in solid tumors through decreasing MHC-I surface expression. Nat Commun 13:3882 (2022). PubMed: 35794100
- Wu J et al. SPOP promotes cervical cancer progression by inducing the movement of PD-1 away from PD-L1 in spatial localization. J Transl Med 20:384 (2022). PubMed: 36042498
- Huo X et al. Identification of Novel Immunologic Checkpoint Gene Prognostic Markers for Ovarian Cancer. J Oncol 2022:8570882 (2022). PubMed: 36157232
- Zhu J et al. Photothermal Nano-Vaccine Promoting Antigen Presentation and Dendritic Cells Infiltration for Enhanced Immunotherapy of Melanoma via Transdermal Microneedles Delivery. Research (Wash D C) 2022:9816272 (2022). PubMed: 36157510