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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD8 aa 200 to the C-terminus (C terminal).
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab93278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
For unpurified use at 1/50 - 1/100.
|WB||1/1000. Predicted molecular weight: 26 kDa.
For unpurified use at 1/1000 - 1/10000.
|IHC-P||1/250 - 1/500.
See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol). This product showed specific staining in lymphocytes although we observed background staining in some epithelial cells tested.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD8 with purified ab93278 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil labelling CD8 with unpurified ab93278 at a dilution of 1/250.