製品の概要

  • 製品名Anti-CD8 antibody [EP1150Y]
    CD8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1150Y] to CD8
  • 特異性The immunogen sequence for this antibody has no significant homology with mouse and rat CD alpha and beta.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD8 aa 200 to the C-terminus (C terminal).

  • ポジティブ・コントロール
    • WB: THP-1 cell lysate. IHC-P: Human tonsil tissue.
  • 特記事項

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500.

For unpurified use at 1/50 - 1/100.

WB 1/1000. Predicted molecular weight: 26 kDa.

For unpurified use at 1/1000 - 1/10000.

IHC-P 1/250 - 1/500.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ターゲット情報

  • 機能Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • 関連疾患Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • 配列類似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻訳後修飾All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 製品の状態CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
  • 別名
    • CD8 antibody
    • CD8 antigen, alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8a antigen antibody
    • CD8a molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    see all

Anti-CD8 antibody [EP1150Y] 画像

  • All lanes : Anti-CD8 antibody [EP1150Y] (ab93278) at 1/5000 dilution (purified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : THP-1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size : 26 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD8 with purified ab93278 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling CD8 with purified ab93278 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Anti-CD8 antibody [EP1150Y] (ab93278) at 1/1000 dilution (unpurified) + THP-1 cell lysate at 10 µg

    Predicted band size : 26 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil labelling CD8 with unpurified ab93278 at a dilution of 1/250.

Anti-CD8 antibody [EP1150Y] (ab93278) 使用論文

This product has been referenced in:
  • Yokoseki A  et al. Hypertrophic pachymeningitis: significance of myeloperoxidase anti-neutrophil cytoplasmic antibody. Brain 137:520-36 (2014). IHC ; Human . Read more (PubMed: 24271323) »
  • Hansen W  et al. Neuropilin 1 deficiency on CD4+Foxp3+ regulatory T cells impairs mouse melanoma growth. J Exp Med 209:2001-16 (2012). Read more (PubMed: 23045606) »

See all 2 Publications for this product

Product Wall

Application Flow Cytometry
Sample Human Cell (peripheral blood mononuclear cells (PBMC))
Permeabilization Yes - 0.5% saponin
Gating Strategy lymphocytes based on FSC vs SSC
Specification peripheral blood mononuclear cells (PBMC)
Fixation Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 13 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Sample Human Tissue sections (Intestine)
Specification Intestine
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 24 2014

Merci de nous avoir contactés.


Je suis désolée de vous avoir raté aujourd’hui. J'ai essayé de vous rappeler 4 fois aujourd'hui sur votre numéro de téléphone 0492038999, mais ...

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Vielen Dank für Ihre Anfrage.

Ich habe Ihnen hier vier Antikörper herausgesucht, die wenig bzw. gar keine Kreuzreaktion mit dem Maus-Protein aufweisen, so dass Sie nur das humane Protein im Mausgewebe detektieren sollten.

Drei der...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (spleen)
Specification spleen
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TE-Buffer
Permeabilization Yes - DAKO Wash Buffer
Username

Mr. Rudolf Jung

Verified customer

投稿 Feb 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"