The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Enumeration and monitoring of the T-suppressor/cytotoxic subset in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
Analysis of cell-mediated cytotoxicity
Analysis of immunoregulation and T-lymphocyte–mediated suppression
Studies of NK lymphocyte subsets.
CD8 (FITC) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD8 (FITC) is sufficient for labelling of 1x106 cells.
Molecular F/P ratio: between 3.0 and 6.0
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells.
CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
Wassmer T et al. The retromer coat complex coordinates endosomal sorting and dynein-mediated transport, with carrier recognition by the trans-Golgi network. Dev Cell17:110-22 (2009).
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