製品の概要

  • 製品名Anti-CD8 antibody [144B]
    CD8 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [144B] to CD8
  • アプリケーション適用あり: IHC-R, WB, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (Human) (13 C terminal amino acids of the cytoplasmic domain).

  • ポジティブ・コントロール
    • Tonsil

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab17147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-R Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • 関連疾患Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • 配列類似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻訳後修飾All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 製品の状態CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
  • 別名
    • CD8 antibody
    • CD8 antigen, alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8a antigen antibody
    • CD8a molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    see all

Anti-CD8 antibody [144B] 画像

  • Human peripheral blood lymphocytes stained with ab17147 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab17147, 1/100 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

  • Formalin fixed paraffin embedded human tonsil stained with CD8 using ABC and DAB chromogen.
  • ab17147 staining human tonsil by IHC-P.
  • ab17147 staining CD8 in human nasal polyp and tonsil tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with 10% formalin for 24 hours and a heat mediated antigen retrieval step was performed using EDTA pH 8.0. Samples were then blocked with 20% DMEM and 1% BSA in culture medium for 20 minutes at 21°C followed by incubation with the primary antibody at a 1/150 dilution, for 18 hours at 4°C. A biotinylated rabbit anti-mouse (Fab2) polyclonal was used as secondary antibody at a 1/400 dilution.
    An Avidin Biotin blocking step was used in addition to those already mentioned.

    See Abreview

Anti-CD8 antibody [144B] (ab17147) 使用論文

This product has been referenced in:
  • Engels CC  et al. Immunological subtypes in breast cancer are prognostic for invasive ductal but not for invasive lobular breast carcinoma. Br J Cancer N/A:N/A (2014). Human . Read more (PubMed: 24937677) »
  • Wheat R  et al. Inflammatory cell distribution in primary merkel cell carcinoma. Cancers (Basel) 6:1047-64 (2014). Read more (PubMed: 24961933) »

See all 3 Publications for this product

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Thank you for contacting us. While we have not tested this product in rabbit samples, BLASTs of the immunogen region show that the immunogen used has only a 67% (CD8A) and 46% (CD8B) homology with rabbit. We consider a baseline homology of 85% ne...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Nasal Polyp and Tonsil)
Specification Nasal Polyp and Tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH8
Permeabilization No
Blocking step 20% DMEM and 1% BSA in culture medium as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Sep 28 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Resin sections)
Sample Human Tissue sections (Nasal polyp and tonsil)
Specification Nasal polyp and tonsil
Username

Abcam user community

Verified customer

投稿 Aug 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin - psoriasis)
Specification skin - psoriasis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH 6.0
Permeabilization Yes - TBST
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Miss. Sandra Peternel

Verified customer

投稿 Apr 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Peripheral T-lymphoyctes, PC3 cells)
Loading amount 20 µg
Specification Peripheral T-lymphoyctes, PC3 cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jan 15 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"