The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5µg for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
ab111919 at 0.5 µg/106 cells detecting CD8 alpha in Fischer Rat thymus cells by Flow Cytometry. 95% more of the cells were stained than in the control (FITC Mouse IgG1, k). Cells from Rat strains Wistar, Buffalo and Brown Norway were also positive in this assay.
Johnson P et al. Purification, chain separation and sequence of the MRC OX-8 antigen, a marker of rat cytotoxic T lymphocytes. EMBO J4:2539-45 (1985).
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Thomas ML & Green JR Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens. Eur J Immunol13:855-8 (1983).
Read more (PubMed: 6416869) »