Anti-CD8 alpha 抗体 [EP1150] (ab108343)

製品の概要

  • 製品名Anti-CD8 alpha antibody [EP1150]
    CD8 alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1150] to CD8 alpha
  • アプリケーション適用あり: IP, IHC-P, ICC, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of Human CD8 alpha.

  • ポジティブ・コントロール
    • MOLT-4, Jurkat cell lysates, Human tonsil tissue
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EP1150
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP 1/10.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • 関連疾患Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • 配列類似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻訳後修飾All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

Anti-CD8 alpha antibody [EP1150] 画像

  • ab108343 at 1/250 dilution staining CD8 alpha in Human tonsil tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

Anti-CD8 alpha antibody [EP1150] (ab108343) 使用論文

ab108343 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (peripheral blood mononuclear cells (PBMC))
Permeabilization Yes - 0.5% saponin
Gating Strategy lymphocytes based on FSC vs SSC
Specification peripheral blood mononuclear cells (PBMC)
Fixation Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 13 2016

Thank you very much for your phone call.

The best antibodies we can recommend are as follows. Please note that the recommendations are based on immunogen sequence only we currently do not hold any rabbit specific data.

Anti CD3 : ab...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Colon)
Specification Colon
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step (agent) for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

投稿 Dec 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"