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Synthetic peptide corresponding to residues near the C-terminus of Human CD8 alpha.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab108343 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
Immunocytochemistry/Immunofluorescence analysis of U-937 cells labelling CD8 alpha with ab108343 at a 1/200 dilution. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were counterstained with ab195889, an Alexa Fluor® 594-conjugated mouse anti-tubulin antibody (1/200). Nuclei counterstained with DAPI (blue).
Secondary antibody only control: PBS instead of primary antibody.
Cytoplasmic staining seen in U-937 cells.
Flow cytometric analysis of paraformaldehyde-fixed human peripheral blood mononuclear cells (PBMC) labeling CD8 alpha with ab108343 at 1/40 dilution, followed by F(ab')2 Frag Goat Anti-Rabbit IgG (H+L) Alexa Fluor® 488.