Anti-CD79a 抗体 [EP3618] (ab79414)

製品の概要

  • 製品名Anti-CD79a antibody [EP3618]
    CD79a 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP3618] to CD79a
  • アプリケーション適用あり: ICC/IF, WB, IHC-P, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide derived from the extracellular region of Human CD79a.

  • ポジティブ・コントロール
    • Raji cell, Daudi cell, human tonsil tissue and human spleen tissue lysates. Raji cells. Human tonsil tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500.
WB 1/5000 - 1/20000. Detects a band of approximately 44-48 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/140. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.
    • 組織特異性B-cells.
    • 関連疾患Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.
    • 配列類似性Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • 翻訳後修飾Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.
    • 細胞内局在Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
    • Information by UniProt
    • 参照データベース
    • 別名
      • B lymphocyte-specific MB1 protein antibody
      • B-cell antigen receptor complex-associated protein alpha chain antibody
      • CD 79a antibody
      • CD79a antibody
      • CD79a antigen (immunoglobulin-associated alpha) antibody
      • CD79A antigen antibody
      • CD79a molecule, immunoglobulin-associated alpha antibody
      • CD79A_HUMAN antibody
      • Ig alpha antibody
      • Ig-alpha antibody
      • IGA antibody
      • IgM-alpha antibody
      • Immunoglobulin-associated alpha antibody
      • Ly54 antibody
      • MB-1 membrane glycoprotein antibody
      • MB1 antibody
      • Membrane-bound immunoglobulin-associated protein antibody
      • Surface IgM-associated protein antibody
      see all

    Anti-CD79a antibody [EP3618] 画像

    • All lanes : Anti-CD79a antibody [EP3618] (ab79414) at 1/10000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Daudi cell lysate
      Lane 3 : Human tonsil tissue lysate
      Lane 4 : Human spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 25 kDa
      Observed band size : 44-48 kDa (why is the actual band size different from the predicted?)
    • ab79414 at 1/100 dilution staining CD79a in paraffin-embedded human tonsil tissue by Immunohistochemistry.
    • Immunocytochemistry/Immunofluorescence analysis of RAMOS (human Burkitt's lymphoma) cells labelling CD79a with purified ab79414 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • Flow cytometric analysis of Raji cells using ab79414 at a 1/140 dilution (red) or a rabbit IgG (negative) (green).
    • ab79414 showing positive staining in Normal spleen tissue.

    • ab79414 showing positive staining in Normal tonsil tissue.

    • ab79414 showing negative staining in Normal brain tissue.

    • ab79414 showing negative staining in Normal colon tissue.

    • ab79414 showing negative staining in Normal heart tissue.

    • ab79414 showing negative staining in Normal brain tissue.

    • ab79414 showing positive staining in Normal tonsil tissue.

    • ab79414 showing negative staining in Normal colon tissue.

    • ab79414 showing negative staining in Normal heart tissue.

    • ab79414 showing positive staining in Normal spleen tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-CD79a antibody [EP3618] (ab79414) 使用論文

    ab79414 has not yet been referenced specifically in any publications.

    Product Wall

    According to our records, ab79414 was proving difficult to use in IHC and we were in contact in order to help resolve the issue.

    Looking at our correspondence, it appears that we are awaiting more details in order to help us better understan...

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    Thank you for contacting Abcam regarding ab79414.


    The localization of this protein should be to the membrane. The only other information we have regarding this is that following antigen binding, the BCR has been shown to translocate fro...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"