製品の概要

  • 製品名Anti-CD75 antibody [LN1]
    CD75 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [LN1] to CD75
  • 特異性This antibody is shown to be a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Tissue/ cell preparation - Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.

  • ポジティブ・コントロール
    • Lymph node or human tonsil.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • 精製度Ammonium Sulphate Precipitation
  • ポリ/モノモノクローナル
  • クローン名LN1
  • アイソタイプIgM
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/25 - 1/50. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Flow Cyt Use 1µg for 106 cells. ab18400-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
  • パスウェイProtein modification; protein glycosylation.
  • 配列類似性Belongs to the glycosyltransferase 29 family.
  • 翻訳後修飾The soluble form derives from the membrane form by proteolytic processing.
    The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme.
  • 細胞内局在Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 6 sialyltransferase antibody
    • 6-sialyltransferase 1 antibody
    • 6-ST 1 antibody
    • Alpha 2 antibody
    • Alpha 2,6 ST 1 antibody
    • Alpha 2,6 ST antibody
    • B cell antigen CD75 antibody
    • B-cell antigen CD75 antibody
    • Beta galactoside alpha 2,6 sialyltransferase 1 antibody
    • Beta-galactoside alpha-2 antibody
    • CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1 antibody
    • CMP-N-acetylneuraminate-beta-galactosamide-alpha-2 antibody
    • MGC48859 antibody
    • Sialyltransferase 1 (beta galactoside alpha 2,6 sialyltransferase) antibody
    • Sialyltransferase 1 antibody
    • SIAT1 antibody
    • SIAT1_HUMAN antibody
    • ST6 beta galactosamide alpha 2,6 sialyltranferase 1 antibody
    • ST6Gal I antibody
    • ST6GAL1 antibody
    • ST6GalI antibody
    • ST6N antibody
    see all

Anti-CD75 antibody [LN1] 画像

  • Formalin-fixed, paraffin-embedded human tonsil stained with ab77676 at 4-8ug/ml, using a peroxidase-conjugate secondary and AEC chromogen. Note cell membrane and cytoplasmic staining of B cells in germinal center.
  • ICC/IF image of ab77676 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77676, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing Ramos cells stained with ab77676 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77676, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-CD75 antibody [LN1] (ab77676) 使用論文

ab77676 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your recent telephone call. I am sorry to confirm that the epitope for ab77676 CD75 antibody [LN1] has regrettably not been mapped. We aim to provide as much information as we can to customers, and I am sorry we do not have ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"