製品の概要

  • 製品名Anti-CD75 antibody [B-L5]
    CD75 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [B-L5] to CD75
  • 特異性This antibody recognises CD75.
  • アプリケーション適用あり: Flow Cyt, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Raji cell line.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 特記事項(精製)This antibody was purified by gel filtration.
  • ポリ/モノモノクローナル
  • クローン名B-L5
  • ミエローマx63-Ag8.653
  • アイソタイプIgM
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab27316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
IP
  • 追加情報Flow Cyt: Use 10 µl to label 106 cells or 100 µl of whole blood.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
    • パスウェイProtein modification; protein glycosylation.
    • 配列類似性Belongs to the glycosyltransferase 29 family.
    • 翻訳後修飾The soluble form derives from the membrane form by proteolytic processing.
      The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme.
    • 細胞内局在Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 6 sialyltransferase antibody
      • 6-sialyltransferase 1 antibody
      • 6-ST 1 antibody
      • Alpha 2 antibody
      • Alpha 2,6 ST 1 antibody
      • Alpha 2,6 ST antibody
      • B cell antigen CD75 antibody
      • B-cell antigen CD75 antibody
      • Beta galactoside alpha 2,6 sialyltransferase 1 antibody
      • Beta-galactoside alpha-2 antibody
      • CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1 antibody
      • CMP-N-acetylneuraminate-beta-galactosamide-alpha-2 antibody
      • MGC48859 antibody
      • Sialyltransferase 1 (beta galactoside alpha 2,6 sialyltransferase) antibody
      • Sialyltransferase 1 antibody
      • SIAT1 antibody
      • SIAT1_HUMAN antibody
      • ST6 beta galactosamide alpha 2,6 sialyltranferase 1 antibody
      • ST6Gal I antibody
      • ST6GAL1 antibody
      • ST6GalI antibody
      • ST6N antibody
      see all

    Anti-CD75 antibody [B-L5] 画像

    • CD75 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to CD75 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

      The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab27316.

      Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

      Band: 47kDa; CD75

    • Ab27316, at a dilution of 10µl for 106 cells or 100 µl of whole blood, staining CD75 by Flow Cytometry.

    Anti-CD75 antibody [B-L5] (ab27316) 使用論文

    ab27316 has not yet been referenced specifically in any publications.

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    Thank you for contacting us. ab99597 is an allophycocyanin conjugated secondary antibody specific to primary Mouse IgM, it would therefore recognise ab27316 (a Mouse IgM). It would not recognise IgG. For more details about secondary antibodies pl...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"