製品の概要
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製品名Anti-CD75 antibody [B-L5]
CD75 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [B-L5] to CD75
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由来種Mouse
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特異性This antibody recognises CD75.
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アプリケーション適用あり: Flow Cyt, IPmore details
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種交差性交差種: Human
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免疫原
Raji cell line.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.1% Sodium Azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
特記事項（精製）This antibody was purified by gel filtration.
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ポリ/モノモノクローナル
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クローン名B-L5
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ミエローマx63-Ag8.653
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アイソタイプIgM
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab27316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | ||
| IP |
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
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パスウェイProtein modification; protein glycosylation.
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配列類似性Belongs to the glycosyltransferase 29 family.
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翻訳後修飾The soluble form derives from the membrane form by proteolytic processing.
The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme. -
細胞内局在Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
- Information by UniProt
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参照データベース
- Entrez Gene: 6480 Human
- Omim: 109675 Human
- SwissProt: P15907 Human
- Unigene: 207459 Human
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別名
- 6 sialyltransferase antibody
- 6-sialyltransferase 1 antibody
- 6-ST 1 antibody
see all
画像
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![Immunoprecipitation - Anti-CD75 antibody [B-L5] (ab27316)](http://a.abcam.com/ps/products/27/ab27316/Images/ab27316-203457-IPVI001ab2731620m.jpg)
Immunoprecipitation - Anti-CD75 antibody [B-L5] (ab27316)CD75 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to CD75 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab27316.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 47kDa; CD75
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![Flow Cytometry - Anti-CD75 antibody [B-L5] (ab27316)](http://a.abcam.com/ps/products/27/ab27316/Images/ab27316.gif)
Flow Cytometry - Anti-CD75 antibody [B-L5] (ab27316)Ab27316, at a dilution of 10µl for 106 cells or 100 µl of whole blood, staining CD75 by Flow Cytometry.
参考文献
ab27316 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"