製品の概要

  • 製品名Anti-CD74 antibody [PIN.1]
    CD74 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [PIN.1] to CD74
  • 特異性This antibody detects an ~33-35 kDa protein doublet, corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74.
  • アプリケーション適用あり: ICC/IF, WB, IP, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    DQKPVMDDQRDLISNNE

    conjugated to KLH, corresponding to amino acids 12-28 of Human CD74.

  • エピトープThe epitope is in a region of the cytoplasmic tail of human CD74 which is common to all isoforms.
  • ポジティブ・コントロール
    • Human B lymphoblastoid cell lysate. IF/ICC: HepG2 cell line.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)This antibody was purified by immunoaffinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名PIN.1
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab22603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa.
IP Use a concentration of 12 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
  • 配列類似性Contains 1 thyroglobulin type-1 domain.
  • 細胞内局在Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 74 antibody
    • CD74 antibody
    • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
    • CD74 antigen antibody
    • CD74 molecule antibody
    • CD74 molecule, major histocompatibility complex, class II invariant chain antibody
    • CLIP antibody
    • DHLAG antibody
    • Gamma chain of class II antigens antibody
    • HG2A_HUMAN antibody
    • HLA class II histocompatibility antigen gamma chain antibody
    • HLA DR antigens associated invariant chain antibody
    • HLA DR gamma antibody
    • HLA-DR antigens-associated invariant chain antibody
    • HLA-DR-gamma antibody
    • HLADG antibody
    • HLADR antigens associated invariant chain antibody
    • Ia antigen associated invariant chain antibody
    • Ia antigen-associated invariant chain antibody
    • Ia associated invariant chain antibody
    • Ia gamma antibody
    • Ii antibody
    • Invariant polypeptide of major histocompatibility complex class II antigen associated antibody
    • la-gamma antibody
    • Major histocompatibility complex class II invariant chain antibody
    • MHC HLA DR gamma chain antibody
    • MHC HLA-DR gamma chain antibody
    • p33 antibody
    • p35 antibody
    • Protein 41 antibody
    see all

Anti-CD74 antibody [PIN.1] 画像

  • ICC/IF image of ab22603 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22603, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Ab22603 staining CD74 in human liver. Staining is localized to the membrane and cytoplasm.
    Left panel: with primary antibody at 4ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (Dako Autostainer plus), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be req
  • Overlay histogram showing Raji cells stained with ab22603 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22603, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-CD74 antibody [PIN.1] (ab22603) 使用論文

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Thank you for contacting us. I would recommend ab22603, which recognizes a very similar epitope (aa12-28) when compared to the antibody you had been using: ab97479 (aa1-28). I hope this helps, please let me know if you need any additional information o...

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Thank you for your reply.

I spoke to the lab and they have told me that they will be unable to produce a large quantity of ab22603 that is glycerol and azide free. Therefore the best way to get this antibody without any of those reagents pres...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"