製品の概要

  • 製品名Anti-CD74 antibody [LN2]
    CD74 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [LN2] to CD74
  • アプリケーション適用あり: Flow Cyt, WB, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    SU-DHL-4 lymphoma cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名LN2
  • ミエローマunknown
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/10. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 2 µg/ml.

ターゲット情報

  • 機能Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
  • 配列類似性Contains 1 thyroglobulin type-1 domain.
  • 細胞内局在Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 74 antibody
    • CD74 antibody
    • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
    • CD74 antigen antibody
    • CD74 molecule antibody
    • CD74 molecule, major histocompatibility complex, class II invariant chain antibody
    • CLIP antibody
    • DHLAG antibody
    • Gamma chain of class II antigens antibody
    • HG2A_HUMAN antibody
    • HLA class II histocompatibility antigen gamma chain antibody
    • HLA DR antigens associated invariant chain antibody
    • HLA DR gamma antibody
    • HLA-DR antigens-associated invariant chain antibody
    • HLA-DR-gamma antibody
    • HLADG antibody
    • HLADR antigens associated invariant chain antibody
    • Ia antigen associated invariant chain antibody
    • Ia antigen-associated invariant chain antibody
    • Ia associated invariant chain antibody
    • Ia gamma antibody
    • Ii antibody
    • Invariant polypeptide of major histocompatibility complex class II antigen associated antibody
    • la-gamma antibody
    • Major histocompatibility complex class II invariant chain antibody
    • MHC HLA DR gamma chain antibody
    • MHC HLA-DR gamma chain antibody
    • p33 antibody
    • p35 antibody
    • Protein 41 antibody
    see all

Anti-CD74 antibody [LN2] 画像

  • ab9514 staining human tonsil by IHC-P.
  • Anti-CD74 antibody [LN2] (ab9514) at 5 µg/ml + Human tonsil normal tissue lysate - total protein (ab29615) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 37 kDa (possible post-translational modification).
  • ab9514 staining CD74 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with neutral buffered formalin and a heat mediated antigen retrieval step was performed using EDTA buffer pH 9.0. Samples were then blocked with 10% serum for 10 minutes at 20°C followed by incubation with the primary antibody at a 1/75 dilution for 30 minutes at 20°C. A HRP-conjugated rat anti-mouse/rabbit polyclonal was used undiluted as the secondary antibody.

    See Abreview

  • Overlay histogram showing Raji cells stained with ab9514 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9514, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-CD74 antibody [LN2] (ab9514) 使用論文

This product has been referenced in:
  • Piao Y  et al. Acquired resistance to anti-VEGF therapy in glioblastoma is associated with a mesenchymal transition. Clin Cancer Res 19:4392-403 (2013). Read more (PubMed: 23804423) »
  • Arenberg D  et al. Macrophage migration inhibitory factor promotes tumor growth in the context of lung injury and repair. Am J Respir Crit Care Med 182:1030-7 (2010). Inhibition Assay ; Mouse . Read more (PubMed: 20581170) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Specification Liver
Fixative Neutral Buffered Formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Dec 29 2010

You could use any unrelated antibody for a negative control, we don't have a specific one to recommend. You can also run a negative control using the same conditions that would be appropriate for antibody staining, replacing the primary antibody with b...

Read More

Thank you for your enquiry. There is no particular negative control tissue for CD74; the negative control should be run on the same tissue (known positive) but without primary antibody. Please contact us again if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"