Anti-CD74 抗体 [EPR4064] (ab108393)

製品の概要

  • 製品名Anti-CD74 antibody [EPR4064]
    CD74 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4064] to CD74
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human CD74.

  • ポジティブ・コントロール
    • Ramos cell lysate; Human tonsil tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR4064
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
IHC-P 1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/150.
ICC/IF 1/500.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
    • 配列類似性Contains 1 thyroglobulin type-1 domain.
    • 細胞内局在Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD 74 antibody
      • CD74 antibody
      • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
      • CD74 antigen antibody
      • CD74 molecule antibody
      • CD74 molecule, major histocompatibility complex, class II invariant chain antibody
      • CLIP antibody
      • DHLAG antibody
      • Gamma chain of class II antigens antibody
      • HG2A_HUMAN antibody
      • HLA class II histocompatibility antigen gamma chain antibody
      • HLA DR antigens associated invariant chain antibody
      • HLA DR gamma antibody
      • HLA-DR antigens-associated invariant chain antibody
      • HLA-DR-gamma antibody
      • HLADG antibody
      • HLADR antigens associated invariant chain antibody
      • Ia antigen associated invariant chain antibody
      • Ia antigen-associated invariant chain antibody
      • Ia associated invariant chain antibody
      • Ia gamma antibody
      • Ii antibody
      • Invariant polypeptide of major histocompatibility complex class II antigen associated antibody
      • la-gamma antibody
      • Major histocompatibility complex class II invariant chain antibody
      • MHC HLA DR gamma chain antibody
      • MHC HLA-DR gamma chain antibody
      • p33 antibody
      • p35 antibody
      • Protein 41 antibody
      see all

    Anti-CD74 antibody [EPR4064] 画像

    • Confocal image of ab108393 staining CD74 in the RAMOS (human Burkitt's lymphoma) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500) and an Alexa Fluor® 488-conjugated goat anti-rabbit  IgG polyclonal (ab150077) was used as the secondary antibody at a dilution of 1/1000.

      DAPI was used ad a nuclear counterstain at 1/200.

    • ab108393 staining CD74 in RAMOS (human Burkitt's lymphoma) cell line. The sample was fixed with 4% paraformaldehyde and incubated with the primary antibody (1/150) for 30 minutes at 4°C. An Alexa Fluor® 488 -conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody.

      Control: Cell without incubation with primary antibody and secondary antibody (Blue).

    • Anti-CD74 antibody [EPR4064] (ab108393) at 1/1000 dilution + Ramos cell lysate at 10 µg

      Predicted band size : 34 kDa
    • Immunohistochemical analysis of CD74 in paraffin embedded Human tonsil tissue, using ab108393 at a 1/100 dilution.
    • ab108393 showing negative staining in Normal brain tissue.

    • ab108393 showing positive staining in B cell lymphoma tissue.

    • ab108393 showing negative staining in Normal placenta tissue.

    • ab108393 showing positive staining in Normal spleen tissue.

    • ab108393 showing negative staining in Normal heart tissue.

    • ab108393 showing negative staining in Normal liver tissue.

    Anti-CD74 antibody [EPR4064] (ab108393) 使用論文

    ab108393 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    The immunogen used to generate ab108393 maps between amino acids 15 and 46 of human CD74 (Swiss Prot: P04233) which is within cytoplasmic domain.

    http://www.uniprot.org/uniprot/P04233

    However, ...

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    Vielen Dank für Ihren Anruf gestern. Ich kann Ihnen nun bestätigen, dass ab108393 (der monoklonale Antikörper aus den Rabbit, über den wir gesprochen haben) ebenfalls mit Citrate Puffer (pH6) als Antigen-Demaskierungspuffer im Labor getestet wurde....

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"