製品の概要

  • 製品名Anti-CD73 antibody - C-terminal
    CD73 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CD73 - C-terminal
  • アプリケーション適用あり: ELISA, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human CD73 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • Human breast carcinoma tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)ab71822 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab71822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 25375632

ターゲット情報

  • 機能Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • 関連疾患Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • 配列類似性Belongs to the 5'-nucleotidase family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

Anti-CD73 antibody - C-terminal 画像

  • Anti-CD73 antibody - C-terminal (ab71822) at 1/100 dilution + Mouse brain tissure lysate at 35 µg

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : 31 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • ab71822 at 1/50 dilution, staining CD73 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-CD73 antibody - C-terminal (ab71822) 使用論文

This product has been referenced in:
  • Clauser L  et al. Selective augmentation of stem cell populations in structural fat grafts for maxillofacial surgery. PLoS One 9:e110796 (2014). ICC/IF ; Human . Read more (PubMed: 25375632) »
  • Kurth TB  et al. Functional mesenchymal stem cell niches in adult mouse knee joint synovium in vivo. Arthritis Rheum 63:1289-300 (2011). Read more (PubMed: 21538315) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"