Anti-CD73 抗体 (ab175396)
Key features and details
- Rabbit polyclonal to CD73
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CD73 antibody
CD73 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CD73 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant full length protein corresponding to Human CD73.
Database link: P21589 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175396の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 63 kDa.
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IHC-P | (4) |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 63 kDa. |
IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. -
関連疾患
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. -
配列類似性
Belongs to the 5'-nucleotidase family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4907 Human
- Entrez Gene: 23959 Mouse
- Entrez Gene: 58813 Rat
- Omim: 129190 Human
- SwissProt: P21589 Human
- SwissProt: Q61503 Mouse
- SwissProt: P21588 Rat
- Unigene: 153952 Human
see all -
別名
- 5' NT antibody
- 5' nucleotidase (CD73) antibody
- 5' nucleotidase precursor antibody
see all
画像
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Paraffin-embedded human placenta stained for CD73 using ab175396 at 1/200 in immunohistochemical analysis.
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All lanes : Anti-CD73 antibody (ab175396) at 1/1000 dilution
Lane 1 : DU145 cell extract
Lane 2 : Mouse liver extract
Lane 3 : Mouse lung extract
Lane 4 : Rat brain extract
Lane 5 : Rat liver extract
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG
Developed using the ECL technique.
Predicted band size: 63 kDa
Exposure time: 30 seconds -
Paraffin-embedded rat spleen stained for CD73 using ab175396 at 1/200 in immunohistochemical analysis.
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Paraffin-embedded human placenta stained for CD73 using ab175396 at 1/200 in immunohistochemical analysis.
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Paraffin-embedded human liver tissue stained for CD73 using ab175396 at 1/200 in immunohistochemical analysis.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (38)
ab175396 は 38 報の論文で使用されています。
- Lee KE et al. General gene expression patterns and stemness of the gingiva and dental pulp. J Dent Sci 17:284-292 (2022). PubMed: 35028049
- Hara T et al. CD73+ Epithelial Progenitor Cells That Contribute to Homeostasis and Renewal Are Depleted in Eosinophilic Esophagitis. Cell Mol Gastroenterol Hepatol 13:1449-1467 (2022). PubMed: 35108658
- Zhu J et al. CD39/CD73 Dysregulation of Adenosine Metabolism Increases Decidual Natural Killer Cell Cytotoxicity: Implications in Unexplained Recurrent Spontaneous Abortion. Front Immunol 13:813218 (2022). PubMed: 35222389
- Joung J et al. CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity. Nat Commun 13:1606 (2022). PubMed: 35338135
- Ishibashi K et al. Single-cell transcriptome analysis reveals cellular heterogeneity in mouse intra- and extra articular ligaments. Commun Biol 5:1233 (2022). PubMed: 36371589