Anti-CD73 抗体 (ab115289)
Key features and details
- Rabbit polyclonal to CD73
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CD73 antibody
CD73 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CD73 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human CD73 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P21589 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term. -
バッファー
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab115289の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/100 - 1/500. Predicted molecular weight: 63 kDa.
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IHC-P | (2) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/100 - 1/500. Predicted molecular weight: 63 kDa. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. -
関連疾患
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. -
配列類似性
Belongs to the 5'-nucleotidase family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4907 Human
- Entrez Gene: 23959 Mouse
- Omim: 129190 Human
- SwissProt: P21589 Human
- SwissProt: Q61503 Mouse
- Unigene: 153952 Human
- Unigene: 244235 Mouse
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別名
- 5' NT antibody
- 5' nucleotidase (CD73) antibody
- 5' nucleotidase precursor antibody
see all
画像
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Immunohistochemistry image of formalin-fixed, paraffin-embedded Human placenta tissue stained with ab115289 at 10 µg followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Anti-CD73 antibody (ab115289) + Mouse brain tissue lysate
Predicted band size: 63 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab115289 は 5 報の論文で使用されています。
- Tripathi A et al. Prognostic significance and immune correlates of CD73 expression in renal cell carcinoma. J Immunother Cancer 8:N/A (2020). PubMed: 33177176
- Meng Y et al. The metabolic syndrome alters the miRNA signature of porcine adipose tissue-derived mesenchymal stem cells. Cytometry A 93:93-103 (2018). PubMed: 28678424
- Eirin A et al. Integrated transcriptomic and proteomic analysis of the molecular cargo of extracellular vesicles derived from porcine adipose tissue-derived mesenchymal stem cells. PLoS One 12:e0174303 (2017). PubMed: 28333993
- Yu YI et al. Ecto-5'-nucleotidase expression is associated with the progression of renal cell carcinoma. Oncol Lett 9:2485-2494 (2015). PubMed: 26137095
- Pagnotta SM et al. Ensemble of gene signatures identifies novel biomarkers in colorectal cancer activated through PPAR? and TNFa signaling. PLoS One 8:e72638 (2013). IHC . PubMed: 24133572