製品の概要

  • 製品名Anti-CD73 antibody [7G2]
    CD73 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7G2] to CD73
  • アプリケーション適用あり: IHC-FoFr, IP, IHC-Fr, Flow Cyt, IHC-P, ICC/IF, ICCmore details
    適用なし: WB
  • 種交差性
    交差種: Human
  • 免疫原

    Full length purified Human placental CD73.

  • ポジティブ・コントロール
    • U266 cell lysates, human tonsil, kidney, and liver tissues, human lymphocytes.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 22164136
IP Use at an assay dependent concentration. Use 10µg per test.
IHC-Fr Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use a concentration of 1 - 2 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration. PubMed: 21612554
ICC/IF Use at an assay dependent concentration. PubMed: 19937263
ICC Use at an assay dependent concentration.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
    • 関連疾患Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
    • 配列類似性Belongs to the 5'-nucleotidase family.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 5' NT antibody
      • 5' nucleotidase (CD73) antibody
      • 5' nucleotidase precursor antibody
      • 5' nucleotidase, ecto antibody
      • 5' nucleotidase, ecto (CD73) antibody
      • 5'-NT antibody
      • 5'-nucleotidase antibody
      • 5NTD_HUMAN antibody
      • CD73 antibody
      • CD73 antigen antibody
      • E5NT antibody
      • Ecto 5' nucleotidase antibody
      • Ecto-5'-nucleotidase antibody
      • eN antibody
      • eNT antibody
      • NT antibody
      • NT5 antibody
      • NT5E antibody
      • NTE antibody
      • Purine 5 Prime Nucleotidase antibody
      see all

    Anti-CD73 antibody [7G2] 画像

    • ab54217 staining human peripheral blood lymphocytes by FACS analysis.
    • ab54217 staining cultured human bone marrow stem cells by ICC/IF. Cells were formaldehyde fixed, permeabilization not done prior to blocking in 1% BSA for 30 minutes at 22°C. The primary antibody was diluted 1/250 and incubated with sample for 12 hours at 4°C. FITC conjugated rabbit polyclonal to mouse diluted 1/1000 was used as the secondary.

    Anti-CD73 antibody [7G2] (ab54217) 使用論文

    This product has been referenced in:
    • Ruiz EJ  et al. A paracrine network regulates the cross-talk between human lung stem cells and the stroma. Nat Commun 5:3175 (2014). IHC-Fr . Read more (PubMed: 24430801) »
    • Häusler SF  et al. Anti-CD39 and anti-CD73 antibodies A1 and 7G2 improve targeted therapy in ovarian cancer by blocking adenosine-dependent immune evasion. Am J Transl Res 6:129-39 (2014). Flow Cyt, Blocking ; Human . Read more (PubMed: 24489992) »

    See all 8 Publications for this product

    Product Wall

    Merci de nous avoir contactés.

    Malheureusement, nous n'avons pas d’hybridome dans notre catalogue.

    Je vous recommande de regarder sur le site Web Biocompare qui vous permettra de rechercher des d'anticorps et leurs fourni...

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    Thank you for contacting us.

    We apologize for this error. The concentration should be read as 1-5ug/ml; the datasheet has been updated.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any mo...

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    I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
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    Thank you for contacting us.

    Apologies for the delay in getting back to you. Unfortunately we do not have another batch of this product available. As I mentioned previously I would be happy to refund the item or issue a free unit of another ...

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    I am sorry for the delay in responding to your request for another batch of ab54217.

    I have been in contact with the lab and unfortunately there is not another lot available. I would be happy to refund the item or issue a free unit of anothe...

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    Thank you for responding to our survey.
    I am sorry to hear the replacement vial did not work as expected. I am currently in contact with the lab to identify whether another batch is available. I will be back in touch as soon as I can. If there is ...

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    DISCOUNT CODE: XXXXXXXXXXXXXXX
    Expiration date: 18-08-2012

    DISCOUNT CODE: XXXXXXXXXXXXXXX
    Expiration date: 18-08-2012

    I am very pleased to hear you would like to accept our offer and test ab11415 and ab11155in [Sheep]. Th...

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    Application Immunocytochemistry/ Immunofluorescence
    Sample Horse Cell (Adipose and bone marrow stem cells)
    Specification Adipose and bone marrow stem cells
    Fixative Paraformaldehyde
    Permeabilization Yes - TRITON x100 0,1%
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
    Username

    Ms. Vega Villar

    Verified customer

    投稿 Mar 29 2012

    Merci de nous avoir contactés et désolé pour le délai de ma réponse.
    Je viens d'être informé que l'anti-CD73 clone [7G2] (référence ab54217) a été décrit pour des tests d'inhibition dans la publication suivante : Thomson LF, et al. Tissue Antigens...

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    Thank you for your reply.

    For dilution of the antibodies ready to use in the experiment, as discussed in the previous email I can suggest to dilute antibodiesin PBS orTBS. It is sometimes helpful to include 0.2% Tween 20, however this may ne...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"