製品の概要

  • 製品名Anti-CD73 antibody [2B6]
    CD73 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2B6] to CD73
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: human CD73 (27-252aa) purified from E. coli

  • ポジティブ・コントロール
    • Cell lysates of human mesenchymal stem cells.
  • 特記事項This antibody was derived from the hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • 関連疾患Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • 配列類似性Belongs to the 5'-nucleotidase family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

Anti-CD73 antibody [2B6] 画像

  • Anti-CD73 antibody [2B6] (ab66953) at 1/1000 dilution + lysates of human mesenchymal stem cells at 30 µg

    Secondary
    goat anti-mouse
    Developed using the ECL technique

    Predicted band size : 63 kDa
    Observed band size : 63 kDa

Anti-CD73 antibody [2B6] (ab66953) 使用論文

ab66953 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step casein solution as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Sample Human Tissue sections (skin)
Specification skin
Permeabilization No
Fixative Acetone
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投稿 Aug 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"