製品の概要

  • 製品名
    Anti-CD73 antibody [1D7]
    CD73 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1D7] to CD73
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, Flow Cyt, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified recombinant fragment of Human CD73 expressed in E. coli.

  • ポジティブ・コントロール
    • Human lung cancer, cholangiocarcinorna, lymph node and esophagus tissues.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 63 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt Use at an assay dependent concentration. PubMed: 23776241

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration. PubMed: 23776241

ターゲット情報

  • 機能
    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • 関連疾患
    Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • 配列類似性
    Belongs to the 5'-nucleotidase family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

Anti-CD73 antibody [1D7] 画像

  • ab91086, at 1/200 dilution, staining CD73 in paraffin-embedded Human lung cancer tissue by Immunohistochemistry using DAB staining.
  • ab91086, at 1/200 dilution, staining CD73 in paraffin-embedded Human cholangiocarcinorna tissue by Immunohistochemistry using DAB staining.
  • ab91086, at 1/200 dilution, staining CD73 in paraffin-embedded Human lymph node tissue by Immunohistochemistry using DAB staining.
  • ab91086, at 1/200 dilution, staining CD73 in paraffin-embedded Human esophagus tissue by Immunohistochemistry using DAB staining.

Anti-CD73 antibody [1D7] (ab91086) 使用論文

This product has been referenced in:
  • Moriguchi T  et al. Gata3 Hypomorphic Mutant Mice Rescued with a Yeast Artificial Chromosome Transgene Suffer a Glomerular Mesangial Cell Defect. Mol Cell Biol 36:2272-81 (2016). IHC ; Mouse . Read more (PubMed: 27296697) »
  • Loi S  et al. CD73 promotes anthracycline resistance and poor prognosis in triple negative breast cancer. Proc Natl Acad Sci U S A 110:11091-6 (2013). WB, IHC-Fr, Flow Cyt ; Human . Read more (PubMed: 23776241) »

See all 2 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH8,5
Sample
Human Tissue sections (appendix)
Specification
appendix
Permeabilization
Yes - Washbuffer from Dako with Tween
Fixative
Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

投稿 Oct 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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