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Tissue/ cell preparation (Mouse) - DBA/2 mouse spleen cells.
The 10-1.D.2 monoclonal antibody blocks binding of CD5 to CD72 on the B cell surface, which leads to inhibition of the positive signal resulting from CD5/CD72 pairing.1 However, 10-1.D.2 can itself trigger a positive signal by binding CD72. This antibody has been shown to be useful in In vitro blocking and proliferation studies.
Our Abpromise guarantee covers the use of ab25320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.|
|Blocking||Use at an assay dependent concentration.|
ab25320 has not yet been referenced specifically in any publications.