製品の概要

  • 製品名
    Anti-CD7 antibody [Huly-m2] (Phycoerythrin)
    CD7 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Huly-m2] to CD7 (Phycoerythrin)
  • 標識
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特異性
    This antibody does not react with granulocytes and B lymphocytes.
  • アプリケーション
    適用あり: Flow Cytmore details
  • 種交差性
    交差種: Human

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    PBS with 0.5% BSA and 0.1% sodium azide
  • Concentration information loading...
  • ポリ/モノ
    モノクローナル
  • クローン名
    Huly-m2
  • ミエローマ
    unknown
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
  • 追加情報
    Diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL) andAcute myeloid leukaemia (CD7+ AML)in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
    For FACS:

    Add 100ml of whole blood to a staining tube.
    To this tube add 10ml of CD7 (PE)reagent.
    Mix gently and incubate 20-30 minutes at 2-8°C.
    Add 2ml of BIO LISANTE(ab1159) at room temperature.
    Vortex tube gently (no more then 2 seconds)then incubate for 10 minutes at room temperature in the dark.
    Wash cells two timeswith 3ml of PBS.
    Resuspend cells in 0.5-1ml of PBS and analyse.

    CD7 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.

    10µl ofCD7 (PE) is sufficient for labelling of 1x106 cells.
    Molecular F/P ratio: 1.0
  • ターゲット情報

    • 機能
      Not yet known.
    • 配列類似性
      Contains 1 Ig-like (immunoglobulin-like) domain.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD7 antibody
      • CD7 antigen (p41) antibody
      • CD7 antigen antibody
      • CD7 molecule antibody
      • CD7_HUMAN antibody
      • GP40 antibody
      • LEU 9 antibody
      • LEU9 antibody
      • p41 protein antibody
      • T cell antigen CD7 antibody
      • T cell leukemia antigen antibody
      • T cell surface antigen Leu 9 antibody
      • T-cell antigen CD7 antibody
      • T-cell leukemia antigen antibody
      • T-cell surface antigen Leu-9 antibody
      • Tp 40 antibody
      • Tp40 antibody
      • TP41 antibody
      see all

    プロトコール

    Anti-CD7 antibody [Huly-m2] (Phycoerythrin) (ab1160) 使用論文

    This product has been referenced in:
    • Porwit-MacDonald A  et al. BIOMED-1 concerted action report: flow cytometric characterization of CD7+ cell subsets in normal bone marrow as a basis for the diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL). Leukemia 14:816-25 (2000). Read more (PubMed: 10803512) »
    • Thurlow PJ  et al. A monoclonal antibody detecting a new human T cell antigen, HuLy-m2. Transplantation 38:143-7 (1984). Read more (PubMed: 6205484) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab1160.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録