製品の概要

  • 製品名
    Anti-CD7 antibody [Huly-m2] (Phycoerythrin)
    CD7 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Huly-m2] to CD7 (Phycoerythrin)
  • 標識
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特異性
    This antibody does not react with granulocytes and B lymphocytes.
  • アプリケーション
    適用あり: Flow Cytmore details
  • 種交差性
    交差種: Human

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    PBS with 0.5% BSA and 0.1% sodium azide
  • Concentration information loading...
  • ポリ/モノ
    モノクローナル
  • クローン名
    Huly-m2
  • ミエローマ
    unknown
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
  • 追加情報
    Diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL) andAcute myeloid leukaemia (CD7+ AML)in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
    For FACS:

    Add 100ml of whole blood to a staining tube.
    To this tube add 10ml of CD7 (PE)reagent.
    Mix gently and incubate 20-30 minutes at 2-8°C.
    Add 2ml of BIO LISANTE(ab1159) at room temperature.
    Vortex tube gently (no more then 2 seconds)then incubate for 10 minutes at room temperature in the dark.
    Wash cells two timeswith 3ml of PBS.
    Resuspend cells in 0.5-1ml of PBS and analyse.

    CD7 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.

    10µl ofCD7 (PE) is sufficient for labelling of 1x106 cells.
    Molecular F/P ratio: 1.0
  • ターゲット情報

    • 機能
      Not yet known.
    • 配列類似性
      Contains 1 Ig-like (immunoglobulin-like) domain.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD7 antibody
      • CD7 antigen (p41) antibody
      • CD7 antigen antibody
      • CD7 molecule antibody
      • CD7_HUMAN antibody
      • GP40 antibody
      • LEU 9 antibody
      • LEU9 antibody
      • p41 protein antibody
      • T cell antigen CD7 antibody
      • T cell leukemia antigen antibody
      • T cell surface antigen Leu 9 antibody
      • T-cell antigen CD7 antibody
      • T-cell leukemia antigen antibody
      • T-cell surface antigen Leu-9 antibody
      • Tp 40 antibody
      • Tp40 antibody
      • TP41 antibody
      see all

    プロトコール

    参考文献

    This product has been referenced in:
    • Porwit-MacDonald A  et al. BIOMED-1 concerted action report: flow cytometric characterization of CD7+ cell subsets in normal bone marrow as a basis for the diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL). Leukemia 14:816-25 (2000). Read more (PubMed: 10803512) »
    • Thurlow PJ  et al. A monoclonal antibody detecting a new human T cell antigen, HuLy-m2. Transplantation 38:143-7 (1984). Read more (PubMed: 6205484) »

    See all 2 Publications for this product

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab1160.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録