特異性This antibody reacts with a glycoprotein of 110 kDa, expressed as intracytoplasmic molecule.
It stains macrophages in a wide variety of tissues.
Myeloid precursor cells and peripheral granulocytes are negative.
This antibody does not react with granulocytes and their precursor cells.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
This antibody is not recommended for frozen sections or cell smears.
機能Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
組織特異性Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
配列類似性Belongs to the LAMP family.
翻訳後修飾N- and O-glycosylated.
細胞内局在Cell membrane and Endosome membrane. Lysosome membrane.