製品の概要

  • 製品名Anti-CD68 antibody [KP1], prediluted
    CD68 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [KP1] to CD68, prediluted
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Rat, Cat, Human, Monkey
    非交差種: Chicken, Dog, Pig
  • 免疫原

    Subcellular fraction of human alveolar macrophages

  • ポジティブ・コントロール
    • Human tonsil, lymph node, or spleen tissue.
  • 特記事項


    ab74704 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained.

製品の特性

  • 製品の状態Prediluted
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.5M Tris HCl, stabilizing protein, pH 7.6
  • Concentration information loading...
  • 精製度Protein G purified
  • 一次抗体 備考ab74704 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained.
  • ポリ/モノモノクローナル
  • クローン名KP1
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

Ready to use.

ターゲット情報

  • 機能Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • 組織特異性Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • 配列類似性Belongs to the LAMP family.
  • 翻訳後修飾N- and O-glycosylated.
  • 細胞内局在Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

Anti-CD68 antibody [KP1], prediluted 画像

  • Formalin-fixed, paraffin-embedded human tonsil stained with ab74704 using peroxidase-conjugate and AEC chromogen. Note intense cytoplasmic staining of macrophages.

Anti-CD68 antibody [KP1], prediluted (ab74704) 使用論文

This product has been referenced in:
  • De Groot CJ  et al. Immunocytochemical characterization of the expression of inducible and constitutive isoforms of nitric oxide synthase in demyelinating multiple sclerosis lesions. J Neuropathol Exp Neurol 56:10-20 (1997). Read more (PubMed: 8990125) »
  • Noorman F  et al. Monoclonal antibodies against the human mannose receptor as a specific marker in flow cytometry and immunohistochemistry for macrophages. J Leukoc Biol 61:63-72 (1997). Read more (PubMed: 9000538) »

See all 4 Publications for this product

Product Wall

J'ai contacté le laboratoire qui m'a confirmé que la concentration d'ab74704 est 1ug/ml.

Thank you for contacting us.

This product is ready to use. Please use the antibody neat on the tissue sections. If in case the background is high, please try 1/10, 1/50 or 1/100 dilutions.

I hope this information is helpful to you...

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Regarding the technique I mentioned when we spoke that utilizes the relative scarcity of isotype subclasses, the page at Dr. Cattoretti's Columbia University page has been removed but a version of it is at this I...

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I am sorry to confirm that as far as we are aware, ab74704CD68 antibody [KP1] has never been tested with samples from rabbit]. All tested species cross-reactivity information is stated on our datasheets, and these...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"