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Tissue/ cell preparation: Rat spleen cells
Our Abpromise guarantee covers the use of ab31630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/50 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
|IHC-Fr||Use at an assay dependent concentration.
Fixation with acetone is recommended.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.|
|RIA||Use at an assay dependent concentration.|
|IHC-FrFl||Use at an assay dependent concentration. PubMed: 25374513|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Alternatively protein digestion with trypsin or pronase prior to IHC protocol could be preformed.|
Staining of rat peritoneal macrophages withAlexa Fluor®647 conjugated Mouse anti Rat CD68, clone ED1 (ab31630) following permeabilisation with Leucoperm.
Immunofluorescence analysis of rat lymph node labelling CD 68 with ab31630. DAPI was used, staining DNA. A green CD4 antibody was used to counterstain. The image was taken at a low magnification. A is an Image of DAPI & ab31630, B is an image of the CD4 counterstain & DAPI, C is merged showing the CD4 counterstain, DAPI & ab31630.
ab31630 staining CD68 in rat liver tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate buffer pH6. Samples were then blocked with 1% BSA for 10 minutes at 21°C followed by incubation with the primary antibody at a 1/800 dilution, for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody at a 1/200 dilution.
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