製品の概要

  • 製品名
    Anti-CD68 antibody [ED1]
    CD68 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [ED1] to CD68
  • 特異性
    Ab31630 recognises a single chain glycoprotein of 110kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68.
  • アプリケーション
    適用あり: Flow Cyt, IHC-Fr, IP, WB, RIA, IHC-FrFl, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Tissue/ cell preparation: Rat spleen cells

  • 特記事項
    This product should be stored undiluted.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    ED1
  • ミエローマ
    Sp2/0-Ag14
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab31630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

Fixation with acetone is recommended.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
RIA Use at an assay dependent concentration.
IHC-FrFl Use at an assay dependent concentration. PubMed: 25374513
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Alternatively protein digestion with trypsin or pronase prior to IHC protocol could be preformed.

ターゲット情報

  • 機能
    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • 組織特異性
    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • 配列類似性
    Belongs to the LAMP family.
  • 翻訳後修飾
    N- and O-glycosylated.
  • 細胞内局在
    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

画像

  • ab31630 staining CD68 in rat liver tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate buffer pH6. Samples were then blocked with 1% BSA for 10 minutes at 21°C followed by incubation with the primary antibody at a 1/800 dilution, for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody at a 1/200 dilution.

    See Abreview

  • Staining of rat peritoneal macrophages withAlexa Fluor®647 conjugated Mouse anti Rat CD68, clone ED1 (ab31630) following permeabilisation with Leucoperm.

  • ab31630 staining CD68 in rat kidney tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate buffer. Samples were then blocked with 10% serum for 1 hour at 4°C followed by incubation with the primary antibody at a 1/200 dilution, for 12 hours at 4°C. A biotin-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody at a 1/100 dilution.

    See Abreview

参考文献

This product has been referenced in:
  • Telegina DV  et al. Contributions of age-related alterations of the retinal pigment epithelium and of glia to the AMD-like pathology in OXYS rats. Sci Rep 7:41533 (2017). ICC/IF ; Rat . Read more (PubMed: 28134357) »
  • Liu H  et al. Jagged1 modulated tumor-associated macrophage differentiation predicts poor prognosis in patients with invasive micropapillary carcinoma of the breast. Medicine (Baltimore) 96:e6663 (2017). IHC-P ; Human . Read more (PubMed: 28422875) »

See all 74 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Tumor)
Permeabilization
Yes - 100% Acetone
Specification
Tumor
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin
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投稿 Aug 02 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Lung)
Permeabilization
Yes - 80% Acetone
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
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投稿 Aug 02 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin
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投稿 May 01 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
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投稿 Apr 17 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9,0
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
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投稿 Jan 19 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Injured spinal cord)
Antigen retrieval step
None
Permeabilization
No
Specification
Injured spinal cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Paraformaldehyde
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投稿 Sep 14 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Microglia)
Permeabilization
Yes - Triton X100
Specification
Microglia
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Verified customer

投稿 Feb 01 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (hepatocytes)
Permeabilization
Yes - 0.1% Triton X-100
Specification
hepatocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative
Paraformaldehyde
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投稿 Dec 15 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M citrate buffer, pH6.0
Sample
Rat Tissue sections (Bladder)
Specification
Bladder
Permeabilization
No
Fixative
Formaldehyde
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投稿 Nov 19 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9,0
Sample
Rat Tissue sections (Intestine)
Specification
Intestine
Permeabilization
No
Fixative
Formaldehyde
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投稿 Nov 07 2014

1-10 of 39 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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