製品の概要

  • 製品名Anti-CD64 antibody [10.1]
    CD64 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [10.1] to CD64
  • アプリケーション適用あり: WB, IP, IHC-P, IHC-Fr, Functional Studies, ICC, Flow Cyt, Blockingmore details
  • 種交差性
    交差種: Human, Non Human Primates
  • 免疫原

    Rheumatoid synovial fluid cells and fibronectin purified Human monocytes.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • バッファーpH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)ab119843 is purified from cell culture supernatant by protein-A affinity chromatography. Purity is > 95% by SDS-PAGE.
  • ポリ/モノモノクローナル
  • クローン名10.1
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab119843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 72 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use a concentration of 5 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Blocking Use at an assay dependent concentration.

ターゲット情報

  • 関連性The high affinity receptor for immunoglobulin G CD64 is encoded by a family of 3 genes that share over 98% of DNA sequence homology: FCGR1A, FCGR1B and FCGR1C. The FCGR1C gene, like FCGR1A, is located on 1q21. The third gene of this family, FCGR1B, was found at 1p12. CD64 plays a pivotal role in the immune response. It is a glycoprotein (MW 72 kD) that is constitutively expressed on human monocytes and macrophages.
  • 細胞内局在Cell membrane; Single-pass type I membrane protein.
  • 参照データベース
  • 別名
    • CD 64 antibody
    • CD64 antibody
    • CD64 antigen antibody
    • CD64A antibody
    • CD64b antibody
    • CD64c antibody
    • Fc fragment of IgG high affinity Ia receptor antibody
    • Fc fragment of IgG high affinity Ib receptor antibody
    • Fc fragment of IgG high affinity Ic receptor antibody
    • Fc fragment of IgG, high affinity Ia, receptor (CD64) antibody
    • Fc fragment of IgG, high affinity Ia, receptor for (CD64) antibody
    • Fc fragment of IgG, high affinity Ia, receptor for antibody
    • Fc fragment of IgG, high affinity Ib, receptor (CD64) antibody
    • Fc fragment of IgG, high affinity Ib, receptor for antibody
    • Fc fragment of IgG, high affinity Ic, receptor (CD64) antibody
    • Fc fragment of IgG, high affinity Ic, receptor (CD64), pseudogene antibody
    • Fc fragment of IgG, high affinity Ic, receptor for antibody
    • Fc gamma receptor antibody
    • Fc gamma receptor I A1 antibody
    • Fc gamma receptor I antibody
    • Fc gamma receptor I B2 antibody
    • Fc gamma RI antibody
    • Fc gamma RIA antibody
    • Fc gamma RIB antibody
    • Fc of IgG high affinity Ia receptor antibody
    • Fc-gamma RIC antibody
    • FCG 1 antibody
    • FCG1 antibody
    • FcgammaRI antibody
    • FcgammaRIa antibody
    • FCGR 1 antibody
    • FCGR1 antibody
    • FCGR1A antibody
    • FCGR1B antibody
    • FCGR1C antibody
    • FcRI antibody
    • FcRIB antibody
    • FCRIC antibody
    • FLJ18345 antibody
    • HFcgammaRIB antibody
    • High affinity immunoglobulin gamma Fc receptor I antibody
    • High affinity immunoglobulin gamma Fc receptor IB antibody
    • IGFR 1 antibody
    • IGFR1 antibody
    • IGFRB antibody
    • IGFRC antibody
    • IgG Fc receptor I antibody
    • IgG Fc receptor IB antibody
    • IgG Fc receptor IC antibody
    • Immunoglobulin G Fc receptor I antibody
    • Immunoglobulin G Fc receptor IB antibody
    • Immunoglobulin G Fc receptor IC antibody
    • MGC137713 antibody
    see all

Anti-CD64 antibody [10.1] 画像

  • Overlay histogram showing THP1 cells stained with ab119843 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab119843, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed THP1 cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-CD64 antibody [10.1] (ab119843) 使用論文

This product has been referenced in:
  • Sánchez-Torres C  et al. CD16+ and CD16- human blood monocyte subsets differentiate in vitro to dendritic cells with different abilities to stimulate CD4+ T cells. Int Immunol 13:1571-81 (2001). Read more (PubMed: 11717198) »
  • Jayaram Y  et al. The Fc receptor, FcRI, and other activation molecules on human mononuclear phagocytes after treatment with interferon-gamma. Clin Exp Immunol 75:414-20 (1989). Read more (PubMed: 2495203) »

See all 3 Publications for this product

Product Wall

I have issued the free of charge antibody ab104273, which should be with you on Monday. The order number is xxxxxx. I hope the suggestions that I have made if not the new antibody will help. I wish you well with your experiments. If you do cont...

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Thank you for contacting us in regards to the problems experienced with ab119843. As we discussed on the phone, I have attached a questionnaire so that I can gather further information regarding the samples tested and the protocol used. Once I rece...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"