Anti-CD63 抗体 [TS63] - BSA and Azide free (ab59479)
Key features and details
- Mouse monoclonal [TS63] to CD63 - BSA and Azide free
- Suitable for: ICC, Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CD63 antibody [TS63] - BSA and Azide free
CD63 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [TS63] to CD63 - BSA and Azide free -
由来種
Mouse -
特異性
Recognises the LIMP antigen, a 30-60 kda (smear) protein. -
アプリケーション
適用あり: ICC, Flow Cyt, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CD63. Jurkat and HEL cell lines.
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ポジティブ・コントロール
- ICC: A431 cells; IHC-P: Human Skin Melanoma; Flow Cyt: HL60 cells;
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Constituent: 100% PBS
Sterile-filtered through 0.22 µm. Carrier and preservative free -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Ion Exchange Chromatography -
特記事項(精製)
Sterile-filtered through 0.22 µm and treated to remove endotoxins. -
ポリ/モノ
モノクローナル -
クローン名
TS63 -
ミエローマ
P3-X63 Ag8.3 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59479の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use a concentration of 5 µg/ml.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IHC-P | (2) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
Use at an assay dependent concentration. Use under non reducing condition. Detects a band of approximately 30-60 kDa (predicted molecular weight: 26 kDa).
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特記事項 |
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ICC
Use a concentration of 5 µg/ml. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Use under non reducing condition. Detects a band of approximately 30-60 kDa (predicted molecular weight: 26 kDa). |
ターゲット情報
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機能
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
組織特異性
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
配列類似性
Belongs to the tetraspanin (TM4SF) family. -
細胞内局在
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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参照データベース
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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別名
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
画像
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ICC image of ab59479 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab59479 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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IHC image of CD63 staining in Human Skin Melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59479, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Overlay histogram showing HL60 cells stained with ab59479 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab59479, 1 µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight®488 goat anti-Mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (218)
ab59479 は 218 報の論文で使用されています。
- Wu Q et al. Presence of Intact Hepatitis B Virions in Exosomes. Cell Mol Gastroenterol Hepatol 15:237-259 (2023). PubMed: 36184032
- Ye Q et al. Association between lncRNAs in plasma exosomes and diabetic retinopathy. Front Endocrinol (Lausanne) 13:987488 (2022). PubMed: 36187126
- Le BCT et al. Characterization and Involvement of Exosomes Originating from Chikungunya Virus-Infected Epithelial Cells in the Transmission of Infectious Viral Elements. Int J Mol Sci 23:N/A (2022). PubMed: 36292974
- Read CB et al. The Obligate Intracellular Bacterial Pathogen Anaplasma phagocytophilum Exploits Host Cell Multivesicular Body Biogenesis for Proliferation and Dissemination. mBio 13:e0296122 (2022). PubMed: 36409075
- Zhao J et al. Mesenchymal Stem Cell-derived Extracellular Vesicles Transmitting MicroRNA-34a-5p Suppress Tumorigenesis of Colorectal Cancer Through c-MYC/DNMT3a/PTEN Axis. Mol Neurobiol 59:47-60 (2022). PubMed: 34623601