製品の概要

  • 製品名Anti-CD63 antibody [NK1/C3]
    CD63 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [NK1/C3] to CD63
  • 特異性This antibody is specific to a 53 kD protein, expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also expressed on granulocytes, B cells and T cells.
  • アプリケーション適用あり: ICC, IHC-P, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Smooth plasma membrane fraction of MeWo cells.

  • 特記事項


    CD63 is expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also present in monocytes and macrophages and is weakly expressed in granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen ME491 and to the platelet antigen PTLGP40.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/100. (Also see PMID 19129916).

ターゲット情報

  • 機能This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
  • 組織特異性Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
  • 配列類似性Belongs to the tetraspanin (TM4SF) family.
  • 細胞内局在Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Lysosomal associated membrane protein 3 antibody
    • CD 63 antibody
    • CD63 antibody
    • CD63 antigen (melanoma 1 antigen) antibody
    • CD63 antigen antibody
    • CD63 antigen melanoma 1 antigen antibody
    • CD63 molecule antibody
    • CD63_HUMAN antibody
    • gp55 antibody
    • Granulophysin antibody
    • LAMP 3 antibody
    • LAMP-3 antibody
    • LAMP3 antibody
    • LIMP antibody
    • Lysosomal-associated membrane protein 3 antibody
    • Lysosome associated membrane glycoprotein 3 antibody
    • Mast cell antigen AD1 antibody
    • ME491 antibody
    • Melanoma 1 antigen antibody
    • Melanoma associated antigen ME491 antibody
    • Melanoma associated antigen MLA1 antibody
    • Melanoma-associated antigen ME491 antibody
    • MGC72893 antibody
    • MLA 1 antibody
    • MLA1 antibody
    • NGA antibody
    • Ocular melanoma associated antigen antibody
    • Ocular melanoma-associated antigen antibody
    • OMA81H antibody
    • PTLGP40 antibody
    • Tetraspanin 30 antibody
    • Tetraspanin-30 antibody
    • Tspan 30 antibody
    • Tspan-30 antibody
    • TSPAN30 antibody
    see all

Anti-CD63 antibody [NK1/C3] 画像

  • ab1318 staining human melanoma by IHC-P.
  • Overlay histogram showing HL60 cells stained with ab1318 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1318, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-Mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-CD63 antibody [NK1/C3] (ab1318) 使用論文

This product has been referenced in:
  • Herfs M  et al. A discrete population of squamocolumnar junction cells implicated in the pathogenesis of cervical cancer. Proc Natl Acad Sci U S A 109:10516-21 (2012). Read more (PubMed: 22689991) »
  • Turner NA  et al. Endothelial cell ADAMTS-13 and VWF: production, release, and VWF string cleavage. Blood 114:5102-11 (2009). ICC/IF ; Human . Read more (PubMed: 19822897) »

See all 9 Publications for this product

Product Wall

Thank you for contacting us. If you are not able to easily pellet the exosomes, I would indeed recommend to add 20 µl of the antibody solution to the exosomes in 100 µl PBS. You may need to increase the incubation time and or the volume of ab81436 a...

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Thank you for contacting us with your question about these antibodies. We don't have exact concentrations for either of these products, but ab1318 is around 100 ug/mL. Since Ab92726 is provided as unpurified tissue culture supernatant, it is not possib...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (cancer)
Specification cancer
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
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Verified customer

投稿 Jun 04 2010

Thank you for your enquiry. The concentration is :100 ug/ml (micrograms per ml). Please do not hesitate to contact us if you need further information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"