Anti-CD62L 抗体 [OX-85] (Phycoerythrin) (ab33924)

製品の概要

  • 製品名Anti-CD62L antibody [OX-85] (Phycoerythrin)
    CD62L 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [OX-85] to CD62L (Phycoerythrin)
  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 特記事項


    In rats, CD62L is expressed by a subset of thymocytes, predominantly single positive CD4 or CD8 cells. Expression of rat CD62L is weak, and sensitive detection systems (for example, three layer biotin-streptavidin techniques) may be required.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab33924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/1 - 1/10. Use 10µl of the suggested working dilution to label 106 cells.



ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
  • 組織特異性Expressed in B cell lines and T lymphocytes.
  • 配列類似性Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A.11 antibody
    • AI528707 antibody
    • CD62 antigen ligand antibody
    • CD62 antigen-like family member L antibody
    • CD62L antibody
    • gp90-MEL antibody
    • IgA nephropathy, susceptibility to, included antibody
    • L Selectin antibody
    • L-selectin antibody
    • LAM-1 antibody
    • LAM1 antibody
    • LECAM1 antibody
    • LEU8 antibody
    • Leukocyte adhesion molecule 1 antibody
    • Leukocyte surface antigen Leu-8 antibody
    • Leukocyte-endothelial cell adhesion molecule 1 antibody
    • Lnhr antibody
    • LSEL antibody
    • Ly-22 antibody
    • Ly-m22 antibody
    • Lyam-1 antibody
    • LYAM1 antibody
    • LYAM1_HUMAN antibody
    • Lymph node homing receptor antibody
    • Lymphocyte adhesion molecule 1 antibody
    • Lymphocyte antigen 22 antibody
    • Lymphocyte surface MEL-14 antigen antibody
    • MEL-14 antibody
    • Pln homing receptor antibody
    • PLNHR antibody
    • Selectin L antibody
    • Selectin, lymphocyte antibody
    • SELL antibody
    • TQ1 antibody
    see all

Anti-CD62L antibody [OX-85] (Phycoerythrin) 画像

  • Overlay histogram showing mouse peritoneal cells stained with ab33924 (red line). Cells were gated on CD3+ cells. The cells were diluted 1/100 in PBS/BSA 1% and incubated for 1 hour at 4ºC. Isotype control antibody (black line) was used under the same conditions.

Anti-CD62L antibody [OX-85] (Phycoerythrin) (ab33924) 使用論文

ab33924 has not yet been referenced specifically in any publications.

Product Wall

Thank you for submitting an Abreview for ab33924. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in an untested species (dog), we would like to follow up on this to see...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Dog Cell (from whole blood)
Specification from whole blood
Preparation Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
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Abcam user community

Verified customer

投稿 Nov 06 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Cynomolgus Monkey Cell (Whole blood)
Specification Whole blood
Preparation Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Apr 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Rat Cell (Splenocytes)
Specification Splenocytes
Preparation Cell harvesting/tissue preparation method: spleen was mashed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization No
Gating Strategy alive lymphocytes
Username

Abcam user community

Verified customer

投稿 Apr 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (cell from peritoneal lavage (after thioglycolate))
Specification cell from peritoneal lavage (after thioglycolate)
Preparation Cell harvesting/tissue preparation method: PBS lavage of the peritoneal cavity
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy CD3+ cells
Username

Abcam user community

Verified customer

投稿 Nov 28 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: Ficoll
Sample buffer: PBS/BSA 1%
Permeabilization No
Gating Strategy CD3+ cells
Username

Abcam user community

Verified customer

投稿 Nov 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"