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Tissue/ cell preparation (Mouse) - C3H/eb cloned mouse B lymphoma 38C-13.
This antibody blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling. It has been used in studies of in vivo and in vitro blocking of adhesion and cytotoxicity studies.
Our Abpromise guarantee covers the use of ab25282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Blocking||Use at an assay dependent concentration.
In vivo and in vitro blocking of adhesion.
|Functional Studies||Use at an assay dependent concentration.
|Flow Cyt||Use 0.1µg for 106 cells.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
Flow cytometry analysis staining CD62L in mouse splenocytes using ab25282 at a dilution of 0.1 μg/106 cells.
ab25282 has not yet been referenced specifically in any publications.