製品の概要

  • 製品名Anti-CD62L antibody [MEL-14] (PE/Cy7 ®)
    CD62L 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [MEL-14] to CD62L (PE/Cy7 ®)
  • 標識PE/Cy7 ®. Ex: 496nm, Em: 774nm
  • 特異性ab25569 recognises CD62L.
  • アプリケーション適用あり: Functional Studies, Flow Cyt, IHC-Fr, IP, Blocking, Inhibition Assaymore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Tissue/ cell preparation: C3H/eb cloned mouse B lymphoma 38C-13.

  • 特記事項This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration. Note: ab25569 can be used for cytotoxicity studies.
Flow Cyt Use 0.1µg for 106 cells. ab154460-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration. Ab25569 can be used to block in vitro binding of lymphocytes to peripheral lymph node HEV.
Inhibition Assay Use at an assay dependent concentration. Ab25569 inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.

ターゲット情報

  • 機能Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
  • 組織特異性Expressed in B cell lines and T lymphocytes.
  • 配列類似性Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A.11 antibody
    • AI528707 antibody
    • CD62 antigen ligand antibody
    • CD62 antigen-like family member L antibody
    • CD62L antibody
    • gp90-MEL antibody
    • IgA nephropathy, susceptibility to, included antibody
    • L Selectin antibody
    • L-selectin antibody
    • LAM-1 antibody
    • LAM1 antibody
    • LECAM1 antibody
    • LEU8 antibody
    • Leukocyte adhesion molecule 1 antibody
    • Leukocyte surface antigen Leu-8 antibody
    • Leukocyte-endothelial cell adhesion molecule 1 antibody
    • Lnhr antibody
    • LSEL antibody
    • Ly-22 antibody
    • Ly-m22 antibody
    • Lyam-1 antibody
    • LYAM1 antibody
    • LYAM1_HUMAN antibody
    • Lymph node homing receptor antibody
    • Lymphocyte adhesion molecule 1 antibody
    • Lymphocyte antigen 22 antibody
    • Lymphocyte surface MEL-14 antigen antibody
    • MEL-14 antibody
    • Pln homing receptor antibody
    • PLNHR antibody
    • Selectin L antibody
    • Selectin, lymphocyte antibody
    • SELL antibody
    • TQ1 antibody
    see all

Anti-CD62L antibody [MEL-14] (PE/Cy7 ®) (ab25569) 使用論文

This product has been referenced in:
  • Quinn KM  et al. Comparative analysis of the magnitude, quality, phenotype, and protective capacity of simian immunodeficiency virus gag-specific CD8+ T cells following human-, simian-, and chimpanzee-derived recombinant adenoviral vector immunization. J Immunol 190:2720-35 (2013). Mouse . Read more (PubMed: 23390298) »
  • Quinn KM  et al. Coadministration of polyinosinic:polycytidylic acid and immunostimulatory complexes modifies antigen processing in dendritic cell subsets and enhances HIV gag-specific T cell immunity. J Immunol 191:5085-96 (2013). ICC/IF . Read more (PubMed: 24089189) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (whole blood)
Specification whole blood
Fixation none
Permeabilization No
Gating Strategy monocyte, lymphocyte and granulocyte
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投稿 May 01 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"